UNLABELLED Label-free LC-MS/MS proteomics has proven itself to be a powerful method for evaluating protein identification and quantification from complex samples. For comparative proteomics, several methods have been used to detect the differential expression of proteins from such data. We have assessed seven methods used across the literature for detecting differential expression from spectral...

The development of liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has made it possible to characterize phosphopeptides in an increasingly large-scale and high-throughput fashion. However, extracting confident phosphopeptide identifications from the resulting large data sets in a similar high-throughput fashion remains difficult, as does rigorously estimating the false di...

Elevated concentrations of progesterone receptor, a sensitive indicator of estrogen responsiveness, have been measured in this study during early serial transplan tation of estrogen-induced renal adenocarcinoma tissue in hamsters. A new approach of tumor transplantation beneath the renal capsule has been used. Fragments of primary tumor tissue transplanted subpannicularly in es trogen-treated h...

introduction: second national medical sciences olympiad was done in shiraz in august 2010 with aim of indentifying scientifically talented individuals, motivating students and orienting extracurricular activities. this olympiad was done in 3 areas, basic sciences, clinical sciences and management. in clinical sciences, we used tosce (team objective structured clinical examination). in this arti...

bronchial leiomyoma is extremely rare. to date less than 60 cases have been reported in the world literature. the presented case here is a 30 year old woman who had been treated for bronchial asthma for several years. her chest radiograph showed bulluos emphysematous changes in the right lung and computer tomography scan found the tumor in the right main bronchus near carina. diagnosis was made...

background: different studies have shown that inadequate and unbalanced intake of nutrients including micronutrient and macronutrient, have negative effects on exercise performance and optimal ability; besides, it decreases physical efficiency in athletes. the present study aimed to assess the nutritional status and body composition of female athletic students in isfahan university of medical s...

Abstract We present integral field spectroscopic observations of the nearby ( z ? 0.03) dual active galactic nuclei (AGNs) Mrk 739, whose projected nuclear separation is ?3.4 kpc, obtained with Multi Unit Spectroscopic Explorer at Very Large Telescope. find that galaxy has an extended AGN-ionized emission-line region extending up to ?20 kpc away from nuclei, while star-forming regions are more ...

Human glycoproteins exhibit enormous heterogeneity at each N-glycosite, but few studies have attempted to globally characterize the site-specific structural features. We have developed Integrated GlycoProteome Analyzer (I-GPA) including mapping system for complex N-glycoproteomes, which combines methods for tandem mass spectrometry with a database search and algorithmic suite. Using an N-glycop...

MOTIVATION Statistical evaluation of the confidence of peptide and protein identifications made by tandem mass spectrometry is a critical component for appropriately interpreting the experimental data and conducting downstream analysis. Although many approaches have been developed to assign confidence measure from different perspectives, a unified statistical framework that integrates the uncer...