excretory-secretory products (esp) play an important role in the host biochemical defense by means of activities of detoxifying and antioxidant enzymes. the aim of this study was to evaluate praziquantel (pzq) effects by detection of glutathione s- transferase (gst) and superoxide dismutase (sod) enzymes activities in esp samples of fasciola spp. fasciola gigantica and fasciola hepatica adult p...

Although schistosomicidal drugs and other control measures exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. In this study, native fatty acid binding protein (FABP) from Fasciola gigantica was purified from the adult worm's crude extract by saturation with ammonium sulphate followed by separation on DEAE-Sephadex A-50 anion exc...

A retrospective study covering a period of 10 years (1990--1999) was conducted using post mortem meat inspection records of the Veterinary Department Information Management Unit at Harare to determine the prevalence and seasonal variation of bovine fasciolosis in Zimbabwe. Records of monthly and annual returns from five major abattoirs were examined in regard to total cattle slaughtered and the...

A glycoprotein (27 kDa) was isolated from crude somatic antigen of Fasciola gigantica by two steps affinity chromatography and was used in early detection of experimental fasciolosis in cattle by indirect ELISA and in dot-ELISA formats. Although, anti-27 kDa antibodies could be detected after 3 weeks post infection (WPI) by dot - ELISA which was one week later than indirect ELISA. The test, dot...

Fasciola gigantica (Digenea) is an important foodborne trematode that causes liver fluke disease (fascioliasis) in mammals, including ungulates and humans, mainly in tropical climatic zones of the world. Despite its socioeconomic impact, almost nothing is known about the molecular biology of this parasite, its interplay with its hosts, and the pathogenesis of fascioliasis. Modern genomic techno...

BACKGROUND The aim of this study was to detect the genotype of Fasciola spp. in Meshkin-Shahr, Ardabil Province, northwestern Iran in different hosts using PCR-RFLP. METHODS The parasite hosts included cattle, and sheep. Overall, 70 adult flukes from livers of slaughtered animals were collected from the abattoirs of aforementioned area. The included 35 samples from infected sheep and 35 sampl...

glutathione s-transferase (gst) represents the major class of detoxification enzymes from helminth parasites such as fasciola hepatica and f. gigantica and it is a candidate for chemotherapeutic and vaccine design. therefore, gst enzyme of fasciola spp. could be a target for evaluation of drugs such as triclabendazole (c14h9cl3n2os). for this purpose, gst enzymes were purified from fasciola spp...

This work was to survey gastrointestinal parasites of cattle and goat slaughtered at Trans Amadi abattoir in Port-Harcourt. A total of 100 faecal samples of the ruminants were examined comprising of 50 cattle and 50 goats. Formol-ether concentration technique was used for sample analyses and examined with microscopically. The data obtained were subjected to simple statistical analysis using per...