BACKGROUND The intracytoplasmic sperm injection procedure ending with total fertilization failure is very distressfull event for both the clinician and the patient. OBJECTIVE The aim of this study was to identify independent factors which could be used to identify total fertilization failure before the day of intracytoplasmic sperm injection. MATERIALS AND METHODS This was a retrospective s...

Decrease in fertility and conception rates is a major cause of economic loss and cow culling in dairy herds. Conception rate is the product of fertilization rate and embryonic survival rate. Identification of genetic factors that cause the death of embryos is the first step in eliminating this problem from the population and thereby increasing reproductive efficiency. A candidate pathway approa...

This study evaluated the effect of melatonin supplementation of in vitro maturation media on in vitro maturation (IVM) and in vitro fertilization (IVF) rate of buffalo oocytes. Cumulus oocytes complexes (COCs) were aspirated from follicles of 2-8 mm diameter. In experiment I, COCs were matured in IVM medium supplemented with 0 (control), 250, 500, and 1000 μM melatonin for 22-24 hours in CO2 in...

The in vitro effect of 17α, 20β-Dihydroxyprogesterone on the maturation of Persian sturgeon (Acipenser persicus) oocytes for 7, 10, 12, 24 and 30th hours of incubation were investigated. Blood serum, testosterone (T), progesterone (P4) and 17-β estradiol concentrations were measured using enzyme-linked immuno-sorbent assay (ELISA). The oocytes were incubated in SIS, RM2, L-15 and PSACF media in...

Meta-analyses have found conflicting results with respect to the use of progesterone or progesterone plus estrogen as luteal phase support for in vitro fertilization (IVF) protocols involving gonadotropins and/or gonadotropin-releasing hormone analogs. The aim of the present study was to perform an updated meta-analysis on the efficacy of progesterone versus progesterone plus estrogen as luteal...

Objectives: 1. To investigate the incidence, isotypes and location of antisperm antibodies (ASA) on the sperm surface in an infertile population. 2. To study the influence of isotypes, location of ASA on the sperm surface on fertilization, cleavage rates of embryos and pregnancy rate. Methods: A prospective analytical study was carried out on infertile couples who underwent Assisted Reproductiv...

objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...

in order to do in vitro fertilization (ivf), 435 oocytes were collected from 20 super ovulated nmri female mice, in m2 media with 4% bsa. 85% (n=370) of collected oocytes were in mii phase. epididymal sperms were added to 332 oocytes in t6 media drops with 4% bsa and incubated. fertilization rate was 79.8% (n=265) out of which 27.5% (n=73) grew to morula and 20.8% (n=55) achieved blastocyst sta...