AlkB is one of four proteins involved in the adaptive response to DNA alkylation damage in Escherichia coli and is highly conserved from bacteria to humans. Recent analyses have verified the prediction that AlkB is a member of the Fe(II) and 2-oxoglutarate (2OG)-dependent oxygenase family of enzymes. AlkB mediates repair of methylated DNA by direct demethylation of 1-methyladenine and 3-methylc...

The purpose of this study was to extend previous observations of a greater decrease in tidal volume in infants than in children during halothane anaesthesia. We analyzed the inspiratory flow waveform recorded during spontaneous ventilation in: infants, two to six months of age, and children, one to five years of age. In addition we analyzed the CO2 signal and the pressure waveform during an occ...

Over the past 10 years, thousands of first-into-human (FIH) clinical trials have been performed in Europe, with few severe adverse events (SAEs). Each has received detailed prior safety review at both the local clinical research facility and at national drug regulatory authority level. The recent fatal SAE in the BIA-102474-101 clinical trial shows the limitations of this process. Although crit...

BACKGROUND microRNA is a small non-coding RNA molecule and functions in RNA silencing and post-transcriptional regulation of gene expression. This study was designed to evaluate the role of miR-98 in the development of microvascular permeability and its molecular pathogenesis. METHODS Forty-eight healthy adult Wistar rats were divided into the control group (n = 8) and burn group (n = 40) tha...