method of fixing samples before antibody staining (6), and it would be interesting to assess the suitability of such samples for FISH. Quantitation of successful FISH in tissue sections is not straightforward because of sample-to-sample variation, nuclear truncation in sectioning and variations across the slide in both tissue density and section width. Accurate comparison of FISH sensitivity fo...

This paper describes our participation at the LifeCLEF Fish task 2015. The task is about video-based fish identification. Firstly, we applied foreground detection method with selective search to extract candidate fish object window. Then deep convolutional neural network is used to classify fish species per window. Classification results are post-processed to produce final identification output...

A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Streptococcus parauberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex ...

Omega-3 rich fish oils are extremely labile, thus requiring control of oxidation and off flavor development. A recently proposed emulsification method, layer-by-layer (LbL) deposition, was found to be a plausible method to enhance the characteristics of bioactive ingredients, especially lipids. The present work was designed to test the possibility of enhancing the uptake and utilization of omeg...

UNLABELLED Urothelial carcinoma is a disease at high risk of recurrence after the initial therapy (70-80%) and with the tendency to progression accomplishing the recurrence (30%). Long lasting monitoring of patients with urothelial carcinoma is necessary. Cystoscopy and cytology are currently the primary modalities used to detect and monitor urothelial carcinoma. However, cytology has relativel...

The walleye population in Canadarago Lake, New York, was 81-95% exploited in the 1988 fishing season, the year in which a previous restriction on the length and number of legally harvestable fish was liberalized. Using diary records from a subset of fishermen, growth estimates, and an estimate of the walleye population in the following year, a method is developed to reconstruct the fish populat...

This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a preenrichment step (37C, 18h). In order to evaluate the efficiency of FISH, 235 naturally c...

Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determ...

Fish is an important source of protein in most countries in the world. The need to know the reproduction and population of fish is crucial for optimum exploitation of fish resources in maintaining the requirement of mankind in the future. In fisheries research, the length of a fish is the main parameter needed to identify fish reproduction, recruitment, growth and mortality. Current method used...