8-Hydroxyquinoline derivative 1 as a fluorescent chemosensor for Zn2+ was synthesized. Because Cd2+ is often found with Zn2+ in the environment and can form fluorescent complexes with chelating fluorophores, a potentially important property of chemosensors for Zn2+ is their selectivity for Zn2+ over Cd2+. The Zn2+ or Cd2+ complexes of 1 gave an emission band from the 1:1 complex, but the fluore...

We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleachi...

This review is confined to sensors that use fluorescence to transmit biochemical information. Fluorescence is, by far, the most frequently exploited phenomenon for chemical sensors and biosensors. Parameters that define the application of such sensors include intensity, decay time, anisotropy, quenching efficiency, and luminescence energy transfer. To achieve selective (bio)molecular recognitio...

This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA-RBP interactions through changes in fluorescence intensity. We an...

Abstract Fluorescent probes are valuable tools to visualize non‐catalytic proteins in live cells. Currently, the majority of imaging reagents for based on “always‐on” fluorophores and use these usually necessitate a wash step remove unbounded before microscope imaging. Herein, we report arylamino‐substituted rhodamine as an activatable fluorophore protein We have shown induction arylamino struc...

The quenched fluorescent intensity of a copper(II) complex, 1, of a fluorescent ligand, in degassed methanol or aqueous (buffered at pH 7.2) solution, was found to reappear on exposure to nitric oxide. Thus, it can function as a fluorescence based nitric oxide sensor. It has been found that the present complex can be used to sense nanomolar quantities of nitric oxide in both methanol and pH 7.2...

Background and Aims: Rubella is predominantly a childhood disease that is endemic throughout the world and when rubella outbreaks occur, they are accompanied by birth defects following congenital rubella syndrome. Immunity to rubella virus as a teratogenic agent has an important role for prevention of these serious congenital defects. Lymphocyte proliferation assay is a way for investigation of...

background and objectives: pseudomonas aeruginosa is one of the most important opportunistic pathogens in patients who suffer from immunodeficiency and burns. wound infections caused by p. aeruginosa can rapidly change to systemic and life threatening situation. the aim of this study was to apply florescent in situ hybridization (fish) method for rapid detection of p. aeruginosa in burrn patien...

We demonstrate a new drug screening method for determining the binding affinity of small drug molecules to a target protein by forming fluorescent gold nanoclusters (Au NCs) within the drug-loaded protein, based on the differential fluorescence signal emitted by the Au NCs. Albumin proteins such as human serum albumin (HSA) and bovine serum albumin (BSA) are selected as the model proteins. Four...

The effects of eight different narrow band-emitting fluorescent lamps (371-750 nm) and four commercial broad band-emitting fluorescent sources upon growth and shoot initiation in tobacco callus (Nicotiana tabacum var. Wisconsin 38) have been characterized. Wavelength and intensity are equally important parameters in determining morphogenic changes. Near ultraviolet light (371 nm) was found to s...