4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 Over the past decade the relatively new Fast Micromethod for detecting DNA damage (strand breaks, alkali-labile sites and incomplete excision repair) and related DNA integrity in cell suspensions or tissue homogenates in single microplates has been developed. The technique is based on the ability of the specific fluorophore PicoG...

A sensitive fluorimetric ELISA was developed for the analysis of aflatoxins. The assay was performed in a 384 microwell plate, wherein high specificity monoclonal antibody against AFM1 (mAb-AFM1) was used as capture antibody and FITC conjugated secondary antibody was used for detection and quantification of the analyte. The linear range of the immunoassay was found to be 6.25-50 pg/mL. AFM1 as ...

The tripeptide Hip-His-Leu was used to standardize a fluorimetric method to measure tissue angiotensin-converting enzyme (ACE) activity in rats. The fluorescence of the o-phthaldialdehyde-His-Leu adduct was compared in the presence and absence of the homogenate (25 microl) to determine whether the homogenate from different tissues interfered with the fluorimetric determination of the His-Leu pr...

Endonuclease activity has been detected in association with highly purified virions, pentons, and/or dodecons of adenovirus types 2, 3, 5, 9, 12, 15, and 16. Only single-strand scissions in substrate DNA were detected. The nuclease activity was detected by a highly sensitive ethidium bromide fluorimetric assay procedure.

A fluorimetric assay of aldehyde dehydrogenase isozymes, based on naphthaldehyde oxidation, is compared with Western Blotting analysis on several clinical samples obtained from surgery. The comparison reveals qualitatively good correlation of ALDH1A1 isozyme detection with two methods and somewhat worse on ALDH3A1 assay.

From the effect of β-cyclodextrin and hydroxypropyl-β-cyclodextrin on the UVvisible and fluorescence spectra of carbaryl and carbofuran, the values of association constants were determined. The ratio of the fluorescence quantum yields for the bound and free substrates indicated an enhanced fluorimetric method of detection.

9-[2-(1,4-Dioxa-7,13-dithia-10-azacyclopentadecyl)phenyl]amino-benzo[b]quinolizinium enables the unambiguous fluorimetric and polarimetric detection of Hg(2+) in the close proximity of double-stranded nucleic acids without interfering background signals from the complexes of this compound with Hg(2+) or DNA alone.