OBJECTIVES The stability of circulating proteins can be affected by repeated freezing and thawing. The aim of our study was to identify the effect of repeated freezing and thawing on the plasma and serum concentrations of eight proteins [interferon-γ, interleukin (IL)-8, IL-15, IL-17A, matrix metalloproteinase (MMP)-7, tumor necrosis factor-α, vascular endothelial growth factor (VEGF), and VEGF...

An enzymatic biotransformation process based on extracted pyruvate decarboxylase (PDC) overcomes the problem of by-product benzyl alcohol production. Seven methods of cells disruption which included application of glass beads, freezing/thawing sequence, silver nanoparticles, ultrasonication, freezing/thawing sequence with glass beads, freezing/thawing sequence with silver nanoparticles, and fre...

OBJECTIVE The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology. DESIGN Experimental study MATERIAL AND METHOD Twenty semen samples were obtained from patients attending the infertility unit. The seme...

Suspensions of leukemic cells of mice from three different strains of leukemia were subjected to rapid or slow freezing and rapid or slow thawing. Suspensions rapidly frozen to -196 degrees C. were in all cases innocuous, whereas those frozen slowly were capable of transmitting leukemia. The infectivity of slowly frozen material varied from an estimated 0.0001 per cent to 1 per cent of that of ...

Abstract—A number of studies have been conducted recently to investigate the influence of randomly oriented fibers on some engineering properties of cohesive and cohesionless soils. However, few studies have been carried out on freezing-thawing behavior of fine-grained soils modified with discrete fiber inclusions and additive materials. This experimental study was performed to investigate the ...

Background: Hen egg yolk is a common component of most semen cryopreservation extenders. However, diluents containing the egg yolk may have adverse effects on the quality of the semen. Egg yolk could be solubilized by the addition of sodium dodecyl sulphate (SDS) in semen extenders. Many studies have shown beneficial effects of antioxidants addition to semen freezing extenders. Antioxidants are...

Projects in seasonal frozen soil areas are often faced with frost heaving and thawing subsidence failure, the foundation fill of most projects is a mixture rock. Therefore, taking soil–rock different rock contents as research objects, residual deformation under multiple freezing–thawing cycles studied. In addition, deep learning method based on artificial neural network was pioneered combined t...

Generally speaking, the literature indicates that the number of bacteria surviving subfreezing temperatures will be functions of: (1) initial numbers of viable cells present when frozen (Record and Taylor, 1953; Kropp, 1950), (2) the rate of the freezing and thawing process (Luyet and Gehenio, 1940), (3) the temperature of storage, with 0 to -20 C being more destructive than the range below -20...