PURPOSE Photochemical cross-linking of corneal collagen is an evolving treatment for keratoconus and other ectatic disorders. We evaluated collagen cross-linking by rose bengal plus green light (RGX) in rabbit eyes and investigated factors important for clinical application. METHODS Rose bengal (RB, 0.1%) was applied to deepithelialized corneas of enucleated rabbit eyes for 2 minutes. The dif...

PURPOSE The biomechanical properties of the cornea have an important role in determining the shape of the cornea and visual acuity. Since the cornea is a nonhomogeneous tissue, it is thought that the elastic properties vary throughout the cornea. We aim to measure a map of corneal elasticity across the cornea. METHODS An acoustic radiation force elasticity microscope (ARFEM) was used to creat...

PURPOSE Assess the lamellar organisation of the peripheral and central stroma of the keratoconus (KC) and normal cornea. METHODS Five normal and three KC corneas were fixed in 2.5% glutaraldehyde and processed for electron microscopy. The ultrathin sections were observed under JEOL 1400 TEM, and digital images were taken with a bottom-mounted 11-megapixel Quamisa camera, using the iTEM softwa...

Topographic analysis systems used to analyse the cornea profile have some limitations due to the aspheric nature of the cornea. In many cases the cornea is modelled by a spherocylindrical surface. However the normal cornea is aspheric and flattened from the center to the periphery. Moreover diseased and postsurgical corneas rarely approximate a spherocylindrical surface. Topographic analysis sy...

There was a partial thickness laceration of the right cornea at the junction of the middle and lower thirds extending in an irregular horizontal line from side to side. From the depth of this laceration the cornea was split to the lower limbus and in the lamellar pocket so formed were embedded multiple fragments of a shattered corneal contact lens. At no point did this lamellar laceration penet...

Keratoconus is a progressive corneal thinning of unknown cause in which the cornea assumes a conical shape, with progressive irregular astigmatism and deterioration of visual acuity. The normal anterior corneal surface is prolate, and it could be described as conic (flattening of the radius of curvature from the apex toward the periphery)1. In keratoconus corneas, the steepening of the central ...