The synthetic pathway for 6-substituted phenyl-2-[{(4'-substituted phenyl-5'-thioxo)-1,2,4-triazol-3-yl}-methyl]-2,3,4,5-tetrahydropyridazin-3-one compounds was achieved by a sequence of reactions starting from respective aryl hydrocarbons and is illustrated in Scheme 1. All the compounds were tested for their in vitro antifungal activity on five fungal species, namely Candida albicans, Trichop...

conclusions potent antifungal activity, make this plant an effective replacement treatment for fungal infections or fungal strains that are resistance to synthetic antifungals. results thirty-two components were identified in vitex agnus-castus eo. the main compound was alpha-pinene (19.48%). the total phenolic content of eo was determined as 82.26 ± 5.94 mg gallic acid equivalent (gae)/g eo. t...

Similarities in fungal and animal cells make antifungal discovery efforts more difficult than those for other classes of antimicrobial drugs. Currently, there are only three major classes of antifungal drugs used for the treatment of systemic fungal diseases: polyenes, azoles, and echinocandins. Even in situations where the offending fungal organism is susceptible to the available drugs, treatm...

the root and flowers of butea monosperma (lam.) were extracted with methanol. extensive chromatographic separation and purification with the organic solvents was done. four phytochemicals were separated and their structures were established based on various spectroscopic techniques. isolated crude extract was subjected for antibacterial activity against gram-negative and gram-positive bacteria ...

pectinases have multiple nature and various forms which are necessary for the hydrolysis of pectin in several conventional industrial and natural processes. in this investigation fourteen fungal strains which isolated from agricultural soils ,were screened for pectinase activity. pectinase activity were measured in vitro by assaying released reducing sugar from pectin as sole carbon source. asp...

Defects in a form of noncanonical autophagy, known as LC3-associated phagocytosis (LAP), lead to increased inflammatory pathology during fungal infection. Although LAP contributes to fungal degradation, the molecular mechanisms underlying LAP-mediated modulation of inflammation are unknown. We describe a mechanism by which inflammation is regulated during LAP through the death-associated protei...

Plants are exposed to a large number of pathogenic fungi. Although they do not have an immune system, plant have evolved a variety of potent defense mechanisms, including the synthesis of low molecular weight compounds, proteins, and peptides that have antifungal activity. These proteins appear to be involved in either constitutive or induced resistance to fungal attack. Generally, these protei...

Fractions obtained from partitioning of aerial methanolic extract of Euphorbia Aellenii with chloroform (E2), ethyl acetate (E3), n-butanol (E4) and water (E5) were investigated in vitro for their phytotoxicity, insecticidal, anti-leishmanial, cytotoxicity, antibacterial and antifungal activities. Sample E2 appeared to have significant phytotoxic activity. In the insecticidal screening study o...

Proteolytic, aminopeptidase, endopeptidase and carboxypeptidase activities of seven strains of rumen anaerobic fungi, selected to represent the fungal population commonly found in the rumen, were investigated in vitro. Whatever the nitrogen source included in the culture medium, a proteolytic activity against the 14C-labelled casein was detected in only one fungal strain. This strain belonged t...

Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF) N-acetyl-beta-D-glucosaminide and MUF beta-D-lactoside were used for the detection and quantifi...