The study aimed at isolating a potential acid protease producing fungal strain from local soil source. The fungal strains were isolated from garden soil on the basis of clearance zone on casein-glycerol agar flooded by coomassie blue stain. Aspergillus sp. showing maximum clearance on casein agar plates (pH 5) was selected for further studies. Optimization of various factors influencing maximum...

Long-term fertilization alters soil microbiological properties and then affects the organic carbon (SOC) pool. However, interrelations of SOC with biological drivers their relative importance are rarely analyzed quantitatively at aggregate scale. We investigated contribution microbial biomass, diversity, enzyme activity to C pool in fractions (>5 mm, 2–5 1–2 0.25–1 <0.25 mm) topsoil (0–15...

Enzymes are proteins that catalyse chemical reactions by lowering the activation energy needed and thus speed up the reaction itself. The advantage of enzymes is that they can be applied under mild reaction conditions and that they exhibit high substrate specificity, stereoselectivity and less side product formation than conventional chemical reactions, making biotechnological processes often m...

Laccase belongs to the small group of enzymes called the blue multi copper oxidases. Laccase is widely distributed in higher plants and fungi. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin. Laccases have been subject of intensive research in the last decades due to their broad substrate specif...

Moonlighting proteins, including metabolic enzymes acting as transcription factors (TF), are present in a variety of organisms but have not been described in higher fungi so far. In a previous genome-wide analysis of the TF repertoire of the plant-symbiotic fungus Tuber melanosporum, we identified various enzymes, including the sulfur-assimilation enzyme phosphoadenosine-phosphosulfate reductas...

Abstract Aims An essential task of agricultural systems is to improve internal phosphorus (P) recycling. Cover crops and tillage reduction can increase sustainability, but it not known whether stimulation the soil microbial community availability organic P pools. Methods In a field experiment in southwest Germany, effects winter cover crop mixture (vs. bare fallow) no-till non-inversion tillage...

Head blight, which is caused by mycotoxin-producing fungi of the genus Fusarium, is an economically important crop disease. We assessed the potential of host-induced gene silencing targeting the fungal cytochrome P450 lanosterol C-14α-demethylase (CYP51) genes, which are essential for ergosterol biosynthesis, to restrict fungal infection. In axenic cultures of Fusarium graminearum, in vitro fee...

The aerosolization process of fungal propagules of three species (Aspergillus versicolor, Penicillium melinii, and Cladosporium cladosporioides) was studied by using a newly designed and constructed aerosolization chamber. We discovered that fungal fragments are aerosolized simultaneously with spores from contaminated agar and ceiling tile surfaces. Concentration measurements with an optical pa...