This paper reports on the evaluation of a high resolution micro ultrasonic machining (HR-μUSM) process suitable for post fabrication trimming of complex 3D microstructures made from fused silica. Unlike conventional USM, the HR-μUSM process aims for low machining rates, providing high resolution and high surface quality. The machining rate is reduced by keeping the micro-tool tip at a fixed dis...

The origin recognition complex (ORC) is conserved in all eukaryotes. The six proteins of the Saccharomyces cerevisiae ORC that form a stable complex bind to origins of DNA replication and recruit prereplicative complex (pre-RC) proteins, one of which is Cdc6. To further understand the function of ORC we recently determined by single-particle reconstruction of electron micrographs a low-resoluti...

In aortic stenosis plasma lipoprotein-derived lipids accumulate in aortic valves. Here, we first compared the lipid compositions of stenotic aortic valves and atherosclerotic plaque cores. Both pathological tissues were found to be enriched in cholesteryl linoleate, a marker of extracellularly accumulated lipoproteins. In addition, a large proportion of the phospholipids were found to contain a...

3D printing for tissue engineering requires biomaterials with mechanical and biological properties suitable both regeneration the process. A filament made of poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) combined 45S5 Bioglass (BG) was used to print scaffolds by fused deposition modeling (FDM). Chemical treatment BG particles chlorotrimethylsilane (CTMS) improved ductility extruded filaments ...

Fused Deposition modelling (FDM) technology is based on decomposition of 3-D computer models into thin cross sectional layers, followed by physically forming the layer and stacking them up layer by layer. FDM provide freedom to add material in the desired area and we are also able to create hollow region in certain portion of layer. In this way low weight with good strength single ply raw mater...

Engineering tissues utilizing biodegradable polymer matrices is a promising approach to the treatment of a number of diseases. However, processing techniques utilized to fabricate these matrices typically involve organic solvents and/or high temperatures. Here we describe a process for fabricating matrices without the use of organic solvents and/or elevated temperatures. Disks comprised of poly...

Surface modification of fused silica windows caused by the laser ablation of surface-bound microparticles is investigated. Using optical and electron microscopies between laser pulses, we detail the ablation, fragmentation and dispersal of 2-150 m diameter particles of various materials. Following complete ablation and ejection of all debris material, surface pitting was found to be highly dep...