The role of H(+) excretion in auxin-induced growth of soybean hypocotyl tissues has been investigated, using tissues whose cuticle was rendered permeable to protons or buffers by scarification (scrubbing). Indoleacetic acid induces both elongation and H(+) excretion after a lag of 10 to 12 minutes. Cycloheximide inhibits growth and causes the tissues to remove protons from the medium. Neutral b...

Blue light (BL) activates the plasma membrane H(+)-ATPase via phosphorylation of the C-terminus with concomitant binding of 14-3-3 protein to the terminus in stomatal guard cells. However, the binding site and role of 14-3-3 protein in this physiological response have not been elucidated. We investigated the above using synthetic phosphopeptides designed from the C-terminus of Vicia H(+)-ATPase...

Abstract The seven 14-3-3 isoforms are highly abundant human proteins encoded by similar yet distinct genes. recognize phosphorylated motifs within numerous and viral proteins. Here, we analyze X-ray crystallography, fluorescence polarization, mutagenesis fusicoccin-mediated modulation the structural basis druggability of binding to four E6 oncoproteins tumorigenic papillomaviruses. bind varian...