Simple protein separation by 1DE is a widely used method to reduce sample complexity and to prepare proteins for mass spectrometric identification via in-gel digestion. While several automated solutions are available for in-gel digestion particularly of small cylindric gel plugs derived from 2D gels, the processing of larger 1D gel-derived gel bands with liquid handling work stations is less we...

We introduce the use of immobilized enzymes in hydrogels for proteomic sample processing in digital microfluidic systems. In this technique, pre-formed cylindrical agarose discs bearing immobilized enzymes were integrated into digital microfluidic devices bearing arrays of electrodes. In some experiments, fluorogenic enzyme substrates were delivered to gel discs which served as integrated micro...

This paper presents GelClust, a new software that is designed for processing gel electrophoresis images and generating the corresponding phylogenetic trees. Unlike the most of commercial and non-commercial related softwares, we found that GelClust is very user-friendly and guides the user from image toward dendrogram through seven simple steps. Furthermore, the software, which is implemented in...

and Implications The ELISA assay is a more rapid and sensitive method for PCR product detection than conventional gel electrophoresis. The PCR/ELISA allows for the processing of numerous samples, is relatively inexpensive and eliminates the need for electrophoretic and photographic equipment as well as the use of potential carcinogens such as ethidium bromide which is utilized in the gel electr...

bioactive glasses are silicate-based and can form a strong chemical bond with the tissues. these biomaterials are highly biocompatible and can form a hydroxyapatite layer when implanted in the body or soaked in the simulated body fluid. due to several disadvantages, conventional glass processing method including melting of glass components, is replaced by sol-gel method with a large number of b...

In this paper, we report the first successful fabrication of dense and optically transparent cadmium tungstate (CWO) films by sol-gel processing and the study of their optical and x-ray scintillation properties. A new sol-gel processing method was developed using tungstic acid and cadmium nitrate as precursors and hydrogen peroxide as solvent; homogeneous and stable CWO sols were aged at room t...

Human eosinophil peroxidase (EPO) was purified from leukocytes obtained from a patient with hypereosinophilia. EPO was extracted from the granule fraction using 0.2 mol/L sodium acetate pH 4.0, and the extract was subjected to gel chromatography on Sephadex G-75 and ion exchange chromatography on Biorex 70. The mol wt calculated from gel chromatography was approximately 50,000. However, under r...

Rietveld analyses of neutron diffraction data collected on a series of Pb(Zr0.5Ti0.5)O3 samples, which had been prepared by sol-gel and conventional mixed oxide methods, showed that each sample contained both rhombohedral and tetragonal PZT (Pb[Zr1-xTix]O3). The zirconium-to-titanium ratio in the two phases was found to be markedly different. Pair distribution functions were also calculated for...

Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues. In this paper, we present a fully automated system with high accuracy for analyzing DNA and proteins. The proposed algorith...

Link for citation: Fedorov K.M., Gilmanov A.Ya., Shevelev A.P. New approach to simulation and efficiency prediction of precipitation gel enhanced oil recovery methods. Bulletin the Tomsk Polytechnic University. Geo Аssets Engineering, 2023, vol. 334, no. 5, рр.85-93. In Rus. Relevance. The problem conformance improvement by technologies application may be associated with mathematical objective ...