conclusions the results of this study demonstrated that the tpasp-padre-truncated orf2 gene cassette and the truncated orf2 gene in recombinant plasmids are successfully expressed in eukaryotic cells. the immunogenicity of the two recombinant plasmids with different formulations will be evaluated as a novel dna vaccine in future investigations. results sequence analysis and bioinformatics studi...

background: laron syndrome, is a disease that treated by igf-1. this protein is a single chain and has three disulfide bonds. people with laron syndrome have low rates of cancer and diabetes, although they appear to be at increased risk of casual death due to their stature. igf-1 is synthesized by many tissues and is secreted from liver as an endocrine hormone which is transmitted to other tiss...

conclusions the gp40/15 gene, which was cloned in the pet28a+, was successfully expressed and produced in e. coli. therefore, this protein can be used in future studies to develop recombinant vaccines and diagnostic kits. methods in this experimental study, the gp40/15 gene sequence was extracted from genbank (no. af155624) and cloned in the pet28a+ plasmid. colony polymerase chain reaction (pc...

An expression vector containing the gene encoding most common isoform Bet v 1.0101 of 1 protein, major birch pollen allergen, was created for a subsequent in prokaryotic system Escherichia coli. Total RNA from collected Belarus used as matrix. The pJC40-Веt obtained using molecular-genetic methods: cloning, ligation, transformation. specificity cloned fragment confirmed by sequencing. During st...

background and aims: since the produced recombinant proteins by molecular genetics techniques commonly have some limitations in the application, chimeric protein are introduced. chimeric proteins have found widespread application for the study of protein folding, structure stability, function and immunogenisity. methods: according to the known immunomodulatory effect and structure of hsp70 mole...

background: plants are among promising and suitable platform systems for production of recombinant biopharmaceutical proteins due to several features such as safety, no need for fermentation, inexpensive investment, and fast and easy scale-up. human insulin is one of the most widely used medicines in the world. up to now different expression systems including escherichia coli, yeast and cho hav...

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...

1.Atcheson, C.L., B. DiDomenico, S. Frackman, R.E. Esposito and R.T. Elder. 1987. Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene. Proc. Natl. Acad. Sci. USA 84:80358039. 2.Cha, J., W. Bishai and S. Chandrasegaran. 1993. New vectors for direct cloning of PCR products [published erratum appears in Gene 1994; 141:149]. Gene 136:369-370. 3.Clark, J.M. 19...