Campylobacter is a leading bacterial cause of human foodborne infections in the United States. Recent studies suggest that the organism is highly prevalent in poultry semen and may contribute to vertical transmission between the breeder hen and offspring. Because Campylobacter requires iron for its growth and survival, the objective of this study was to determine if the addition of natural and ...

OBJECTIVE The aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or Bioxcell®) on cryopreservation of buffalo semen. MATERIALS AND METHODS In this experimental study, split pooled ejaculates (n=4), possessing more than 70% visual sperm motility were divided in two aliquots and diluted in Bioxcell® and tris-citric ...

We introduce and analyze greedy equilibria (GE) for the well-known model of selfish network creation by Fabrikant et al. [PODC’03]. GE are interesting for two reasons: (1) they model outcomes found by agents which prefer smooth adaptations over radical strategychanges, (2) GE are outcomes found by agents which do not have enough computational resources to play optimally. In the model of Fabrika...

We introduce and analyze greedy equilibria (GE) for the well-known model of selfish network creation by Fabrikant et al. [PODC’03]. GE are interesting for two reasons: (1) they model outcomes found by agents which prefer smooth adaptations over radical strategychanges, (2) GE are outcomes found by agents which do not have enough computational resources to play optimally. In the model of Fabrika...

The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...

The cryoprotective effects of glycerol in three different semen freezing extenders, Tris-citrate (TRIS), TEST, and Tes-Tris-Egg yolk (TTE), on wild-type (WT) rhesus monkey (Macaca mulatta) sperm cryopreservation have been tested. Sperm motility and viability were examined to evaluate the integrity of frozen-thawed sperm, and the best extender was selected to cryopreserve sperm from transgenic H...

This experiment wascarried out to explore the effect of use of combinations of permeating cryoprotectant and non permeating cryoprotectant on thequality of frozen-thawed Beluga (Huso huso) spermas compared to the use of permeating cryoprotectant as a control. For this work methanol (MeOH) and dimethyl sulfoxide (DMSO)are used aspermeatingcryoprotectantsand bovine serum albuminas a non permeatin...

The objective of this study was to evaluate the efficacy of EK extender alone or incorporation with CoenzymeQ10 (CoQ10) and/or soyphosphatidylcholine (SPC) in poultry semen and their effects on seminal traits during temporal storage at 40C for different time intervals (12 h, 24 h, and 36 h). Heterospermic pooled semen samples diluted (1:4) with EK, EK + SPC, EK+ CoQ10 and EK + SPC + CoQ10 exten...

On-stud assessment of stallion sperm quality can be problematic. A new instrument, the Nucleocounter SP-100, was validated for measuring stallion sperm concentration and viability. It was subsequently used to evaluate sperm viability in Kenney's extender and INRA96. There was a strong correlation between sperm concentrations measured by the Nucleocounter SP-100 and by the Bürker counting chambe...

Slow-cooled stallion spermatozoa, with and without seminal plasma removed by centrifugation, were diluted in Kenney's extender (KE) containing nonfat dry skim milk with glucose and antibiotics or in KE supplemented by adding a modified high-potassium Tyrode's medium (KMT). Four ejaculates from each of four stallions were collected and divided factorially across these four treatments. Percentage...