نتایج جستجو برای: genomic dna of rye
تعداد نتایج: 21229200 فیلتر نتایج به سال:
abstract accumulation of gene changes and chromosomal instability in response to cellular dna damage lead to cancer. dna damage induces cell cycle checkpoints pathways. checkpoints regulate dna replication and cell cycle progression, chromatin restructuring, and apoptosis. checkpoint kinase 2 (chk2) is activated in response to dna lesions. atm phosphorylate chk2. the activated chk2 kinase can p...
Neospora caninum is a coccidian protozoan that causes abortion in dairy and beef cattle and neurological disorders in dogs and horses. To identify N. caninum oocysts in the dog feces the molecular approaches are known as sensitive methods that specifically detect the oocysts. In present study, a polymerase chain reaction (PCR) targeting N. caninum specific Nc5 genomic fragment was performed to ...
background : sequence-based identification of various microorganisms including archaea, bacteria, cyanobacteria, diatoms, fungi, and green algae necessitates an efficient and reproducible genome extraction procedure though which a pure template dna is yielded and it can be used in polymerase chain reactions (pcr). considering the fact that dna extraction from these microorganisms is time consu...
Supernumerary B chromosomes are optional additions to the basic set of A chromosomes, and occur in all eukaryotic groups. They differ from the basic complement in morphology, pairing behavior, and inheritance and are not required for normal growth and development. The current view is that B chromosomes are parasitic elements comparable to selfish DNA, like transposons. In contrast to transposon...
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
The 0AL[0RS wheatÐrye chromosomal translocation originally found in {Amigo| wheat possesses resistance genes for stem rust\ powdery mildew and greenbug biotypes B and C\ but also has a negative e}ect on wheat processing quality[ Recently\ a second 0AL[0RS translocation carrying Gb5\ a gene conferring resistance to greenbug biotypes B\ C\ E\ G and I\ was identi_ed in the wheat germplasm line {GR...
Four sourdoughs (A to D) were produced under practical conditions, using a starter obtained from a mixture of three commercially available sourdough starters and baker's yeast. The doughs were continuously propagated until the composition of the microbiota remained stable. A fungi-specific PCR-denaturing gradient gel electrophoresis (DGGE) system was established to monitor the development of th...
Mašková E., Paulíčková I., Rysová J., Gabrovská D. (2011): Evidence for wheat, rye, and barley presence in gluten free foods by PCR method – comparison with ELISA method. Czech J. Food Sci., 29: 45–50. A method of the evidence for the presence of wheat, rye, and barley in gluten free foods, based on the polymerase chain reaction (PCR), was validated. DNA was isolated from foods by chaotropic so...
roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...
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