A heavy chain component of gammaG-globulins in normal urines has the characteristics of the proteolytic digestion product, Fc-fragment. This fragment is estimated to compose up to 15% of the gamma globulins present in urines. In contrast to urinary light chains, the urinary Fc-like fragment probably represents a catabolic component in normal gammaG-globulin metabolism. The light chains of the u...

Cells dissociated from embryonic chick muscle tissue using trypsin were rotated in the presence of globulin-enriched rabbit antisera against both smooth and striated muscle actomyosins originating from chicken gizzard (GAM) and pectoralis (PAM) muscles respectively. The presence of the rabbit antibodies was demonstrated using peroxidase-labelled sheep antirabbit y-globulins, the enzyme-antibody...

19S anti-gamma-globulins were isolated in a high state of purity from the sera of two patients with rheumatoid arthritis. Following reduction with ethyl mercaptan and alkylation by iodoacetamide, fragments were produced which retained the capacity to combine with 7S gamma-globulin. The fragments from one of the 19S anti-gamma-globulins agglutinated red cells coated with incomplete anti-Rh antib...

Various ammonium sulfate concentrations and reaction conditions were employed in the fractionation of sera from rabbits, sheep, horses, and goats. Precipitates and supernatant fluids were analyzed by electrophoresis to study the effects of the controlled variables. At room temperature, the third precipitate in 35% saturated (NH(4))(2)SO(4) was the best fraction from both rabbit and sheep sera; ...

When human and rabbit 7S gamma-globulins were reduced in strong urea solutions by a number of procedures, their molecular weights fell to approximately (1/3) of the original values. Partial separation of the reduction products was achieved using chromatography and starch gel electrophoresis in urea solutions. One of the components of reduced human 7S gamma-globulin was isolated by chromatograph...

Mono-specific antisera against Vibrio cholera Ogawa NIH-43 and Vibrio cholera Inaba 35-A3 were prepared from rabbit hyperimmune sera by absorbing against a heterologous strain. Using ammonium sulphate precipitation procedure, gamma globulins were purified and concentrated. To visualize antigen-antibody reaction, gamma globulins were conjugated to Staphylococcus aureus cowan-l (NCTC: 8325) ...

WO cases of genetic linkage have been established in pigs. The C and J bloodgroup loci are closely linked (ANDRESEN and BAKER 1964; RASMUSEN 1965; ANDRESEN 1966a), and IMLAH (1965) has detected a close linkage between the H p locus for hematin-binding serum proteins (KRISTJANSSON 1961 ; HESSELHOLT 1963b) and the K blood-group locus. This report deals with additional information about the linkag...

This paper describes research into concentration of total proteins and individual protein fractions in the blood serum of Ross broiler chickens during fattening period. Blood for analysis was taken at the 14th, 28th and 42nd days of age by wing vein puncture. Concentration of total serum proteins was detected by spectrophotometry. Electrophoresis on gelled cellulose-acetate tapes determined the...