Dynamic interactions between intracellular networks regulate cellular homeostasis and responses to perturbations. Targeted therapy is aimed at perturbing oncogene addiction pathways in cancer, however, development of acquired resistance to these drugs is a significant clinical problem. A network-based computational analysis of global gene expression data from matched sensitive and acquired drug...

Electrophysiological recordings and calcium measurements in striatal large aspiny interneurons in response to combined O2/glucose deprivation. The effects of combined O2/glucose deprivation were investigated on large aspiny (LA) interneurons recorded from a striatal slice preparation by means of simultaneous electrophysiological and optical recordings. LA interneurons were visually identified a...

D-Glucose deprivation of primary rat brain glial cell cultures, by incubation with 25 mM D-fructose for 24 h, resulted in a 4-5-fold induction of D-glucose transport activity. In contrast, 24-h D-glucose starvation of primary rat brain neuronal cultures had only a marginal effect (1.5-2-fold) on D-glucose transport activity. Northern blot analysis of total cellular RNA demonstrated that under t...

The aim of the study is to evaluate scheme complex therapy based on enteral nutrition and L-arginine, glutamic acid indicators protein, carbohydrate, lipid metabolism in blood rats with experimental wound defects skin under conditions partial food deprivation.
 Materials methods. A biochemical was carried out 24 sexually mature male rats, which were partially deprived for 10 days. On 10th ...

The main energy source powering the brain is glucose. Strong energy needs of our nervous system are fulfilled by conveying this essential metabolite through blood via an extensive vascular network. Glucose then reaches brain tissues by cell uptake, diffusion and metabolization, processes primarily undertaken by astrocytes. Deprivation of glucose can however occur in various circumstances. In pa...

Astrocytes are important for protecting neurons in the central nervous system. It has been reported that some antioxidants could protect astrocytes from ischemia/reperfusion-induced dysfunction. Cinnamtannin B-1 is a naturally occurring A-type proanthocyanidin that exhibits anti-oxidant properties. In this study, we investigated the effects of cinnamtannin B-1 on spinal cord astrocytes. Astrocy...

During starvation or carbohydrate deprivation certain changes take place in the metabolic pattern of animals and human beings, leading to "abnormalities" in carbohydrate metabolism. These changes reveal themselves in manifold ways: the glucose tolerance curve after carbohydrate deprivation is high and prolonged (1-4), and after severe starvation the RQ response to administered glucose is low or...

Although lactic acidosis is a prominent feature of solid tumors, we still have limited understanding of the mechanisms by which lactic acidosis influences metabolic phenotypes of cancer cells. We compared global transcriptional responses of breast cancer cells in response to three distinct tumor microenvironmental stresses: lactic acidosis, glucose deprivation, and hypoxia. We found that lactic...

background: fasting is an alternative method to induceanorexia. objectives: the biochemical factors of liver andserum and clinical signs were measured and documented in fivenon-lactating, non-pregnant cows following eight days of fasting.methods:five crossbred, non-lactating, and non-pregnantcattle were used in this study. they were fasted for 8 days_ totalfood deprivation; however, they had fr...

The role of methionine supplementation in fish metabolism remains largely unexplored. This study investigates the effects of methionine deprivation (MD) on glucose uptake in rainbow trout (Oncorhynchus mykiss) hepatocytes. To this end, primary hepatocytes were incubated in the presence (+M) or the absence (-M) of methionine for 48h and glucose uptake was assessed using a novel non-radioactive, ...