Due to their unique physicochemical properties, carbon nanochips (CNCs) have been used for studies of the direct electrochemical and electrocatalytic properties of oxidoreductase. In this report, a glassy carbon electrode (GCE) was modified with CNCs, and glucose oxidase (GOx) was immobilized on the modified electrode surface. Chitosan (CS) was employed to fix the GOx/CNCs tightly to the surfac...

A glucose sensor was developed by electrocopolymerization using pyrroles containing a tris-bipyridine (bpy) osmium complex (Os-py), pyrrole (py), pyrrole propanoic acid (PPA) and glucose oxidase (GOx) to improve the key performance characteristics, such as the sensitivity, selectivity, and long-term stability. Tris-bipyridine osmium pyrrole complexes with four different methylene moieties were ...

Corresponding Author: Christina Schmid, Ph.D., Institut für Diabetes-Technologie Forschungsund Entwicklungsgesellschaft mbH an der Universität Ulm, Helmholtzstrasse 20, 89081 Ulm, Germany; email address [email protected] Many people with diabetes routinely measure their blood glucose (BG) on capillary blood samples from the fingertip. Beside other interfering factors, the blood sample...

Extracellular and intracellular reactive oxygen species attack different targets and may, therefore, result in different forms of oxidative stress. To specifically study an oxidative stress induced by a regulated intracellular flux of a defined reactive oxygen species in endothelium, we used immunotargeting of the H2O2-generating enzyme glucose oxidase (GOX) conjugated with an antibody to plate...

This paper describes the development of a novel glucose biosensor through layer-by-layer technique (LbL). The self-assembled architectures were composed positive-charged silsesquioxane polyelectrolyte, 3-n-propylpyridinium chloride (SiPy+Cl−), nickel (II) tetrassulphophthalocyanine (NiTsPc), and conductive surface FTO (fluor tin oxide). construction was influenced by isoelectric point (pI) oxid...

Immobilisation of horseradish peroxidase (HRP) and glucose oxidase (GOX) via covalent attachment of modified enzyme carbohydrate to the exterior of the cowpea mosaic virus (CPMV) capsid gave high retention of enzymatic activity. The number of enzymes bound per virus was determined to be about eleven for HRP and 2-3 for GOX. This illustrates that relatively large biomacromolecules can be readily...

This article reports on a study on fluorescence adenosine triphosphate (ATP) detection by InP/ZnS quantum dots (QDs). We present a spectroscopic analysis displaying the effect of enzymatic reactions of glucose oxidase (GOX) and hexokinase (HEX) on the InP/ZnS quantum dots at physiological pH. The InP/ZnS quantum dots act as glucose sensors in the presence of GOX, Glu and ATP, and their luminesc...

Titanium dioxide (TiO2) and Fe3O4 magnetite particles were coated on spherical Kissirises; glucose oxidase (GOx) enzyme was immobilized on Kissiris/Fe3O4/TiO2 by physical adsorption. This catalyst was analyzed by a scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray (EDX) measurements. The performance of the prepared biocatalyst in the...

A hierarchical porous metal-organic framework (MOF), PCN-888, containing three types of cavities was utilized to couple two enzymes into a tandem nanoreactor. The largest cavity (6.2 nm) can only accommodate one molecule of glucose oxidase (GOx). The intermediate cavity (5.0 nm) can accommodate one and only one molecule of horseradish peroxidase (HRP). The small cavity (2.0 nm) has sufficient s...

Co-precipitation of enzymes in metal-organic frameworks is a unique enzyme-immobilization strategy but challenged by weak acid-base stability. To overcome this drawback, we discovered that Ca2+ can co-precipitate with carboxylate ligands and under ambient aqueous conditions form [email protected] material composites stable wide range pHs (3.7–9.5). We proved on four varied isoelectric points, m...