To determine the effects of age and sex on the regulation of postprandial glucose metabolism, glucose turnover, insulin secretion, insulin action, and hepatic insulin extraction were concurrently measured in 145 healthy elderly (aged 70 +/- 1 years) and in 58 young (aged 28 +/- 1 years) men and women before and after ingestion of a mixed meal containing [1-(13)C]glucose. At the time of meal ing...

Carbohydrate consumption during exercise enhances endurance performance. A food-focused approach may offer an alternative, ‘healthier’ given the potential health concerns associated with artificial fructose sources, but food-based carbohydrate sources increase gastrointestinal (GI) symptoms. This study compared cycling performance and GI comfort of two different (fruit artificial) ingested exer...

We previously reported [Xie, Li, Méchin and van de Werve (1999) Biochem. J. 343, 393-396] that dietary phosphate deprivation for 2 days up-regulated both the catalytic subunit and the putative glucose-6-phosphate translocase of the rat liver microsomal glucose-6-phosphatase system, suggesting that increased hepatic glucose production might be responsible for the frequent clinical association of...

The steady state kinetic mechanisms for glucose-6-P dehydrogenase from lactating rat mammary glands were derived for the NADP-linked and NAD-linked reactions. Initial velocity studies and inhibition patterns using NADPH and glucosamine-6-P are consistent with sequential mechanisms with random order addition of glucose-6-P and coenzyme. No evidence was found for enzyme isomerization or dead-end ...

McGinn, S., P. Poronnik, M. King, E. D. M. Gallery, and C. A. Pollock. High glucose and endothelial cell growth: novel effects independent of autocrine TGF1 and hyperosmolarity. Am J Physiol Cell Physiol 284: C1374–C1386, 2003.FirstpublishedJanuary22,2003;10.1152/ajpcell.00466. 2002.—Human endothelial cells were exposed to 5 mM glucose (control), 25 mM (high) glucose, or osmotic control for 72 ...

A gastric [U-14C]glucose load (4.8 mg/g body wt.) was delivered to unrestrained post-absorptive or 30 h-starved rats bearing peripheral and portal vein catheters and continuously perfused with [3-3H]glucose, in order to compare their metabolic and hormonal responses. In the basal state, portal and peripheral glycaemia were less in starved rats than in rats in the post-absorptive period (P less ...