The glycation of protein and nucleic acids that occurs as a consequence of hyperglycemia disrupts cell function and contributes to many pathologies, including those associated with diabetes and aging. Intracellular glycation occurs after the generation of the reactive 1,2-dicarbonyls methylglyoxal and glyoxal, and disruption of mitochondrial function is associated with hyperglycemia. However, t...

The dual mission of the Wisconsin Initiative for Science Literacy is to promote literacy in science, mathematics and technology among the general public and to attract future generations to careers in research, teaching and public service. At the March 5, 2010 UW-Madison Chemistry Department Colloquium, the director of the Wisconsin Initiative for Science Literacy (WISL) encouraged all Ph.D. ch...

The dual mission of the Wisconsin Initiative for Science Literacy is to promote literacy in science, mathematics and technology among the general public and to attract future generations to careers in research, teaching and public service. At the March 5, 2010 UW-Madison Chemistry Department Colloquium, the director of the Wisconsin Initiative for Science Literacy (WISL) encouraged all Ph.D. ch...

Unusual thermostable and insensitive high-energy compounds which contain dioximes with donor–acceptor H-bond sites, were synthesized from glyoxal (40% in water) straightforward steps.

Glyoxal oxidase is produced by ligninolytic cultures of the white-rot fungus Phanerochaete chrysosporium and is a source of the extracellular H2O2 that is required by ligninolytic peroxidases. We report here the cloning and characterization of glx-1c cDNA, which encodes glyoxal oxidase. The deduced mature protein has 537 amino acids, a molecular size of 57 kDa, and a pI of 5.1. Five potential N...

Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. PSBL-1 is a mutant of this organism that generates the ligninolytic system under nonlimiting conditions during primary metabolism. Lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities for PSBL-1 under nonlimiting conditions were 4- to 10-fold higher...

Certain aromatic amidines have been reported to be useful chemotherapeutic agents for the treatment of leishmaniasis, trypanosomiasis, and babesiasis (1). Pharmacological and clinical studies of these compounds have been handicapped by lack of suitable methods for the determination of aromatic amidines in plasma and urine. Recently Fuller (2) described a method which is based upon a reaction be...