An oxidant/antioxidant imbalance is thought to play an important role in the pathogenesis of chronic obstructive pulmonary disease (COPD). We hypothesized that antioxidant capacity reflected by erythrocyte glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities, and serum levels of the lipid peroxidation product malondialdehyde (MDA), may be related to the severit...

Cutaneous squamous cell carcinomas (CSCC) are a common malignancy of keratinocytes that arise in sites of the skin exposed to excessive UV radiation. In the present study, we show that human SCC cell lines, preneoplastic solar keratoses (SK), and CSCC are associated with perturbations in glutathione peroxidase (GPX) activity and peroxide levels. Specifically, we found that two of three SKs and ...

p53-mediated apoptosis may involve the induction of redox-controlling genes, resulting in the production of reactive oxygen species. Microarray expression analysis of doxorubicin exposed, related human lymphoblasts, p53 wild-type (WT) Tk6, and p53 mutant WTK1 identified the p53-dependent up-regulation of manganese superoxide dismutase (MnSOD) and glutathione peroxidase 1 (GPx). Consensus p53 bi...

Esworthy, R. Steven, Jeffrey R. Mann, Mindy Sam, and Fong-Fong Chu. Low glutathione peroxidase activity in Gpx1 knockout mice protects jejunum crypts from g-irradiation damage. Am J Physiol Gastrointest Liver Physiol 279: G426–G436, 2000.—Gpx1 knockout (KO) mice had a higher number of regenerating crypts in the jejunum than did Gpx2-KO or wild-type mice analyzed 4 days after $10 Gy g-irradiatio...

UNLABELLED Aims/Introduction:  We have previously reported that the Pro198Leu missense polymorphism in the glutathione peroxidase 1 (GPx-1) gene was associated with frequent macrovascular disease (MVD). Our goal was to examine whether the GPx-1 genotype is associated with diabetic neuropathy. MATERIALS AND METHODS   We determined the GPx-1 genotype in 173 Japanese type 2 diabetic patients who...

Acetaminophen (AP) is widely used as the antipyretic and analgesic drug in clinic, and it can induce serious liver injury in the case of excessive abuse. The present study showed that AP (400 mg/kg) induced obvious liver injury, while in male mice the hepatotoxicity induced by AP was much more serious than in female mice as indicated by the results of alanine aminotransferase (ALT) activity and...

Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx...

The activity of glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST) was investigated in liver and kidney of rats exposed to cadmium (Cd) and ethanol (EtOH) alone and in combination. Rats were treated with 50 mg Cd/dm3 in drinking water and/or 5 g of EtOH/kg body wt/24 h intragastrically, for 12 weeks. Exposure to Cd led to an increase in GPx and GST acti...

OBJECTIVE This study was designed to determine the relationship between Mycobacterium avium subspecies paratuberculosis (MAP) infection and selenium-dependent glutathione peroxidase (GPx) activity, in the blood of humans and cattle infected with MAP. DESIGN MAP infection status and GPx activity were determined in sera from 42 cattle, a group of 27 patients with Crohn's disease and 27 of their...