Introduction: Glycoprotein B (gB) is the major antigen for induction of humoral responses against human cytomegalovirus (HCMV) making it an attractive candidate for immune prophylaxis. In the present study, the humoral immune response of BALB/c mice to a truncated HCMV gB protein fused with GFP was evaluated. Methods: The truncated gB coding sequence was synthesized and cloned in pEGFPN1 eukary...

Abstract The neurotrophin Brain-derived neurotrophic factor (BDNF) is encoded by multiple bipartite transcripts. Each BDNF transcript composed one out of 11 alternatively spliced exons containing the 5′untranslated region (UTR), and common exon encompassing coding sequence (CDS) 3′UTR with two variants (short long). In neurons, mRNA have a distinct subcellular distribution, constituting “spatia...

The green fluorescent protein (GFP) from Aequorea victoria is a versatile reporter protein for monitoring gene expression and protein localization in a variety of cells and organisms. Despite many early successes using this reporter, wild type GFP is suboptimal for most applications due to low fluorescence intensity when excited by blue light (488 nm), a significant lag in the development of fl...

Detection of azide-tagged biomolecules (e.g., azido sugars) inside living cells using “click” chemistry has been revolutionary to the field chemical biology. However, we currently still lack suitable synthetic biology tools autonomously and rapidly detect azide ions. Here, have developed an engineered promoter system called cyn regulon, complementary Escherichia coli strains, selectively ions i...

Genetic engineering of plants has turned out to be an attractive approach produce various secondary metabolites. Here, we attempted kynurenine, a health-promoting metabolite, in Nicotiana tabacum (tobacco) transformed by Agrobacterium tumefaciens with the gene, coding for human indoleamine 2,3-dioxygenase 1 (IDO1), enzyme responsible kynurenine production because tryptophan degradation. The pre...

Diversification of protein sequence-structure space is a major concern in protein engineering. Deletion mutagenesis can generate a protein sequence-structure space different from substitution mutagenesis mediated space, but it has not been widely used in protein engineering compared to substitution mutagenesis, because it causes a relatively huge range of structural perturbations of target prot...

Green fluorescent protein (GFP) is a protein that emits green fluorescence when exposed to a radiation of ultraviolet wavelength range, even without the addition of substrate and cofactor. Because of such characteristics, the usage of GFP is widespread in both in vivo and in vitro applications. In addition, recent advances in biotechnology have enabled GFP to be expressed in various hosts, incl...

Green fluorescent protein (GFP) has served as a versatile tool in cell biology for studying gene expression, as well as protein folding and trafficking (reviewed in Ref. 1). The utility of GFP in research applications derives from its intrinsic ability to generate fluorescence in live tissues, in the absence of any cofactor(s). By far the most frequent applications of GFP have involved its use ...

Purpose The purpose of this study was to investigate the disease-causing mutations for retinitis pigmentosa (RP) patients and function of mutations. Methods We recruited RP families and sporadic RP patients, and performed whole-exome sequencing (WES) to screen for sequence variations. Subsequently, we investigated the expression of green fluorescent protein (GFP) merged expression vectors con...

The zebrafish embryo is especially valuable for cell biological studies because of its optical clarity. In this system, use of an in vivo fluorescent reporter has been limited to green fluorescent protein (GFP). We have examined other fluorescent proteins alone or in conjunction with GFP to investigate their efficacy as markers for multi-labeling purposes in live zebrafish. By injecting plasmid...