Some antibacterial agents have been shown to neutralize the biological properties of bacterial lipopolysaccharide (LPS). The aim of this study was to elucidate the role of gentamicin, tobramycin, imipenem, tigecycline, and isoniazid in affecting the production of nitric oxide (NO) induced by LPS in mice. Groups of mice were injected intraperitoneally with LPS alone, antibacterial agent alone, o...

The aim of the present paper was to analyze the influence of sampling and storage procedures on nitrite concentration values in the saliva of healthy persons. The samples were obtained and stored under varied conditions, and processed using the Griess method. Results: when the salivary nitrite concentration was measured immediately after collection a significant dependence on the collection tim...

The concept of amplitude-modulated multiplexed flow analysis has been extended to the simultaneous determination of multiple analytes in a sample. A sample solution containing nitrite and nitrate ions is delivered from two channels, but the flow rates are varied at different frequencies. One of the channels has a reduction column for converting nitrate ions to nitrite ions. Downstream, the abso...

A fast and efficient automated method using a sequential injection analysis (SIA) system, based on the Griess, reaction was developed for the determination of nitrate and nitrite in infant formulas and milk powder. The system enables to mix a measured amount of sample (previously constituted in the liquid form and deproteinized) with the chromogenic reagent to produce a colored substance whose ...

Hydroalcoholic extract (70% ethanol extract) of tubers of Momordica tuberosa Cogn. (Cucurbitaceae) was subjected to preliminary phytochemical screening by qualitative tests. Nitric oxide scavenging activity was performed by Griess reagent method. And nephroprotective activity was assessed in gentamicin, cisplatin and paracetamol induced renal damage in wistar rats (150-200 g) by standard method...

Nitrate and nitrite ions were found to be successfully electroreduced at an in situ electrochemically pretreated copper electrode in acidic media, and their reduction peaks of a cyclic voltammogram were found at two distinct electrode potentials. Cyclic voltammetric experiments revealed a highly sensitive behavior of the pretreated copper electrode upon the electroreduction of nitrate and nitri...

OBJECTIVE To study the mechanisms of the antitumor and immunoregulation functions of polyporus polysaccharide (PPS). METHODS The production of nitric oxide (NO), the activity and mRNA expression of inducible nitric oxide synthase (iNOS) in peritoneal macrophages of mice administered with different dose of PPS were observed by Griess reaction, fluorimetry assay and RT-PCR, respectively. RESU...

Nitric oxide (NO) level in serum and renal tissue has been examined in 15 male Wistar rats, body weight 200-250 g, 7 days after unilateral nephrectomy. All rats were ether-anaesthetized and the kidneys were removed by dorsolateral approach. NO concentration in serum and renal tissue was determined by classic colorimetric Griess reaction. Conversion of NO(3)(2-) into NO(2)(2-) was done with elem...

A continuous-flow method for the determination of nitrate and phosphate in water is proposed. The method is based on insertion of reagents and sample solutions into a single channel through a programmable switching valve. The method depends upon heteropolyacid with phosphate and on the modified Griess reaction for nitrate, and permits the determination of PO(-3) (4) in the range 1-20 mug/ml and...

Confirmation of the biological effectiveness new ophthalmic preparations introduced in market is an important element maintaining safety using this type medications. This study aimed to investigate activity Ozodrop® on human corneal and conjunctival epithelial cells, as well its antibacterial antifungal activity. Cytotoxicity analyses ocular surface cells were performed vitro by MTT (3-(4,5-Dim...