A method with the combined advantages of soil particle washing, selective inhibitors, and an indicator substrate was developed to isolate saprophytic basidiomycetes from soil. Organic particles were washed from soil and plated on a medium containing lignin, guaiacol, and benomyl, which reduced mold growth and allowed detection of basidiomycetes producing laccase or peroxidase. The 64 soil sampl...

To investigate the lignification process and its physiological significance under drought-stressed conditions, the changes in enzymes responsible for lignification and the related physiological parameters were determined in white clover (Trifolium repens L.) leaves during 28 d of water deficit treatment. Water deficit gradually decreased leaf water potential (Psiw) to -2.33 MPa at day 28. For t...

In this study peroxidase was isolated from peels of Citrus reticulata var. Kinnow and partially purified using ammonium sulphate precipitation method. Kinetic and thermal characterization of the partially purified enzyme was explored. The optimum pH and temperature were found to be 6 and 55 C respectively. The Km and Vmax values for guaiacol as substrate were found out to be 0.66 mM and 380 μmo...

Chloroperoxidase and horseradish peroxidase use NaClOz as both the oxidant and the halogen donor for the peroxidative chlorination of monochlorodimedone. Previous studies have shown that both horseradish peroxidase and chloroperoxidase can catalyze iodination reactions with hydrogen peroxide as the oxidant; however, only chloroperoxidase catalyzes chlorination reactions under these conditions. ...

Abstract Tomato ( Solanum lycopersicum L.) belongs to the crops that are most sensitive continuous lighting (CL). We studied age-dependent changes in sensitivity of tomato leaves CL. The exposed CL from a lag-phase their growth exhibited pronounced chlorosis with loss 30% chlorophyll after 2 weeks. values maximum F v / m ) and actual (? II quantum yields PSII photochemical activity were decreas...

The purpose of this study is to develop a selective and differential medium (SK2 agar) for isolating guaiacol producing Alicyclobacillus. Forty-one selected dyes and vanillic acid were incorporated in SK agar for screening selective and differential agents. Two guaiacol producing (1016, 1101) and two non-guaiacol producing (19220, C-GD 1-1) Alicyclobacillus isolates were streaked onto media and...

We previously demonstrated that overexpression of the horseradish (Armoracia rusticana) peroxidase prxC1a gene stimulated the growth rate of tobacco (Nicotiana tabacum) plants. Here, the cauliflower mosaic virus 35S::prxC1a construct was introduced into hybrid aspen (Populus sieboldii x Populus grandidentata). The growth rate of these transformed hybrid aspen plants was substantially increased ...

Royal palm tree peroxidase (RPTP) has been isolated to homogeneity from leaves of Roystonea regia palm trees. The enzyme purification steps included homogenization, (NH4)SO4 precipitation, extraction of palm leaf colored compounds and consecutive chromatography on Phenyl-Sepharose, TSK-Gel DEAE-5PW and Superdex-200. The novel peroxidase was characterized as having a molecular weight of 48.2 ± 3...

In a previous paper (1) a calorimetric phenol oxidase method was presented which utilizes catechol as a mediator and in which the oxidation of a leucoindophenol is followed photometrically over a very short time interval. During the development of this method it was found that the same basic method and technique were applicable to the determination of peroxidase. In the latter case, oxidation o...