Proteoglycans were extracted from Swarm rat chondrosarcoma tissue with solutions of 0.5 M guanidine hydrochloride containing protease inhibitors at pH 5.8 and 7.4. In this associative solvent, the noncovalent interactions between the components of the proteoglycan aggregates are not dissociated before subsequent isolation steps. Aggregates were purified by density gradient centrifugation and di...

The yolk precursor protein vitellogenin was isolated from plasma of estrogen-treated Xenopus laevis males, and the yolk platelet proteins, lipovitellin and phosvitin, were isolated from oocytes of females previously stimulated with human chorionic gonadoptropin. The molecular weights of the polypeptide chains of vitellogenin and lipovitellin were determined by electrophoresis on sodium dodecyl ...

Abstract In this paper, a comparative study was conducted on the extraction effects of six agents for collagen-based mural painting binders. These were used to extract residual proteins in non-aged and thermal aged samples. The protein efficiencies different extracting quantitatively determined by bicinchoninic acid (BCA) method, then processed multivariate analysis variance (MANOVA). impact pr...

This study aimed to investigate the dependence of biocidal activity polyguanidine (co)polymers on their structure during formation biofilms by active PE-degrading cultures model microorganisms. The Bc-2 copolymer methacryloyl guanidine hydrochloride (MGHC) and diallyldimethylammonium chloride (DADMAC), which suppressed both growth planktonic cultures, exhibited highest activity. When PE was exp...

The kinetics of the refolding reaction of ribonuclease A from high concentrations of guanidine hydrochloride or urea are biphasic, and show two refolding reactions whose rates differ 450-fold at pH 5.8 and 25 degrees. Measurements of cytidine 2'-phosphate binding during refolding, after stopped-flow dilution of guanidine hydrochloride (Gdn.HCl) or urea, show that functional bovine pancreatic ri...

Marine organisms are prolific resources of guanidine-containing natural products with intriguing structures and promising biological activities. These molecules have therefore attracted the attention of chemists and biologists for their further studies towards potential drug leads. This review focused on the guanidine alkaloids derived from marine sources and discussed the recent progress on th...

The mechanism by which the aqueous cosolvents guanidinium chloride and urea denature proteins is a matter of controversy. Here, we use all-atom molecular dynamics simulations to study the effect of both denaturants on the dewetting of water confined between nanoseparated hydrophobic plates. It is found that the denaturants inhibit the onset of dewetting, so that it occurs at shorter interplate ...