Type III interferons (IFN-λs) signal through a heterodimeric receptor complex composed of the IFN-λR1 subunit, specific for IFN-λs, and interleukin-10Rβ (IL-10Rβ), which is shared by multiple cytokines in the IL-10 superfamily. Low affinity of IL-10Rβ for cytokines has impeded efforts aimed at crystallizing cytokine-receptor complexes. We used yeast surface display to engineer a higher-affinity...

Fevicordin-A (FevA) isolated from Phaleria macrocarpa (Scheff) Boerl. seeds was evaluated for its potential anticancer activity by in vitro and in silico approaches. Cytotoxicity studies indicated that FevA was selective against cell lines of human breast adenocarcinoma (MCF-7) with an IC50 value of 6.4 µM. At 11.2 µM, FevA resulted in 76.8% cell death of T-47D human breast cancer cell lines. C...

Estrogen Receptor (ER) is an important target for pharmaceutical design. Like other ligand-dependent transcription factors, hormone binding regulates ER transcriptional activity. Nevertheless, the mechanisms by which ligands enter and leave ERs and other nuclear receptors remain poorly understood. Here, we report results of locally enhanced sampling molecular dynamics simulations to identify di...

The human papillomavirus type 11 (HPV-11) L1 major capsid protein can be trypsinized to generate recombinant capsomeres that retain HPV genotype-restricted capsid antigenicity (M. Li, T. P. Cripe, P. A. Estes, M. K. Lyon, R. C. Rose, and R. L. Garcea, J. Virol. 71:2988-2995, 1997). In the present study, HPV-11 virion-neutralizing monoclonal antibodies H11.F1 and H11.H3, previously characterized...

background: the development and progression of human tumors is accompanied by various cellular biochemical and genetic alterations. these events include tumor cells interaction with extracellular matrix molecules including hyaluronan and hyaluronan binding protein (ha-habp). hyaluronan is a large polysaccharide associated with pericellular matrix of proliferating, migrating cells, regulation of...

Due to the complexity and uncertainty in the process, the soft computingmethods such as regression analysis, neural networks (ANN), support vector regression (SVR), fuzzy logic andmulti-gene genetic programming (MGGP) are preferred over physics-based models for predicting the process performance. The model participating in the evolutionary stage of the MGGP method is a linear weighted sum of se...

In a second best environment, the optimal policy choice sometimes follows first best rules, as described in a number of scattered works. This paper presents a formal general argument which allows to unify much of the literature. It lays down the information structure and separability assumptions under which the results hold in a variety of setups, with extensions to preference heterogeneity and...

We examine a new dataset of public procurement laws, practice, and outcomes in 187 countries. measure regulation as restrictions on the discretion procuring entities. find that laws practice are highly correlated with each other across countries, better is outcomes, but themselves not outcomes. A closer look shows stricter correlate improved only countries low sector capacity. present model whi...