An economically-important trait in poultry for which gene identification continues to be a challenge is immune response. The objective of the study was quantitate expression major histocompatibility complex (MHC) class II BLB2 at cytolytic and latent response stages Nigerian indigenous chickens. A total 108 chickens (NIC) were sourced across South-western states Nigeria. The birds inoculated...

Newcastle disease (ND) is a of high economic importance to poultry farmers in Nigeria. Its impact on include illness poultry, reduction egg production, immunosuppression, and death. This study was carried out determine the prevalence indigenous (local) chickens from 2 abattoirs Ilorin metropolis. A total 400 blood samples were aseptically collected plain bottles jugular veins local at slaughter...

The serological tests for rheumatoid serum factors fall into two categories, in which rabbit antibody and human gamma globulin respectively provide the reactant. Clinically, the chief use of these tests for serum antiglobulin factors is to assist diagnosis of rheumatoid arthritis, and serum titres above given levels are commonly regarded as "positive". In most tests the "positive" level has bee...

Reverse passive haemagglutination, a novel microtitre based assay, was compared with the Streptex (Wellcome UK) latex slide agglutination kit for streptococcal grouping in a diagnostic microbiology laboratory. Three hundred and fifty two extracts from 349 consecutive primary isolation plates were assayed by both methods. Reverse passive haemagglutination gave identical grouping results for 98.0...

Surfactant protein D (SP-D) is a lung-specific protein that is synthesized and secreted by lung epithelial cells and is believed to play an important role in lung host defence. This protein belongs to the C-type lectin family, which is characterized by an N-terminal cysteine-rich domain, a collagen-like domain, a neck domain and a carbohydrate recognition domain (CRD). To elucidate the biologic...

A total of ninety-eight wild birds, comprising eleven different species were captured alive from Zaria environs. These birds were used for the study, to determine the possible role they play as reservoirs of blood parasites. Thin blood smears (TBS) were prepared from the blood samples collected from these wild birds. The slides were stained and viewed under the microscope for the identification...

The objective of this study was to evaluate and compare the safety, tolerability and immunogenicity for two seasonal influenza subunit vaccines, one with MF59 adjuvant (Fluad) and one without an adjuvant (Agrippal). A total of 195 subjects aged > or = 65 years were enrolled to receive one dose of vaccine intramuscularly, 96 were vaccinated with Fluad, 99 received Agrippal. Blood samples were ta...

BACKGROUND Knowledge of the age-specific prevalence of seroprotection and incidence of seroconversion infection is necessary to complement clinical surveillance data and statistical models. It provides the basis for estimating the future impact of influenza A (H1N1pdm09) and implementing appropriate prevention and response strategies. METHODS Using a cross-sectional design, two-stage stratifi...

Haemagglutination of bluetongue virus (BTV) was demonstrated for the first time by making use of a purified preparation of the virus. The reaction was found to be independent of variations in the pH, temperature, buffer system and origin of the erythrocytes used in the test. A haemagglutination inhibition test, subsequently developed, was demonstrated to be serotype specific. The storage of the...