Injection of trehalose into the haemolymph of Locusta nymphs reduced the amount of grass eaten in one meal. Greater effects were produced by higher concentrations and most reduction occurred when meals began 20 min after injection. Sodium chloride, glycine, proline, sorbose, inulin or a mixture of solutes had similar effects. The similarity of effects is explicable in terms of the increase in h...

Induction of haemolymph proteins in mosquito A. stephensi due to wounding or bacterial infection (E. coli) was analyzed using SDS-PAGE. Wounding response of pupa revealed subsequent induction of two polypeptides (21 and 74 kDa). Two other polypeptides (44 and 57 kDa) were induced commonly in both pupa and adult female haemolymph upon bacterial infection. In vitro binding assay revealed identifi...

Synthesis and release of vitellogenin in the crustacean Isopoda, Porcellio dilatatus, were maximal during premolt. At that time, diurnal variations occurred in the haemolymph protein level and in the synthesis and release of vitellogenin and other proteins in the haemolymph. The pattern of variations was always identical and bimodal: the minima were at dusk and at dawn when the maxima occurred ...

The ionic composition of the haemolymph was measured in fourth instar larvae of Aedes aegypti (reared in 0-30% sea water, the animals' natural salinity range). Inorganic ions and free amino acids form a substantial part of the haemolymph osmotic concentration. With increasing external osmotic pressure, there is an increase in the concentration of the amino acids, not the major inorganic cations...

A number of female sterile mutations on the first and third chromosomes of Drosophila melanogaster have been screened for defects in the yolk proteins using polyacrylamide gel electrophoresis. Two new mutants were identified. 6m45 accumulates all three yolk proteins (YP1, YP2 and YP3) in the haemolymph but they are all absent from the ovaries suggesting it is a yolk-protein-uptake mutant. In co...

During the final larval instar of the tobacco hornworm the presence of juvenile hormone (JH) inhibits the secretion of the brain's prothoracicotropic hormone (PTTH). The corpora allata cease to secrete JH when the larvae attain a weight of approximately 5 g. The JH is cleared from the haemolymph in about 24 h. This process in itself renders the brain competent to release PTTH. The actual releas...

It has been shown by Waddington (1959) that larvae of Drosophila tnelanogaster (Oregon K) can be selected over several generations to survive and develop on a medium containing 7 % NaCl. As few species are known that can survive in such concentrated media, it was considered of interest to study the haemolymph composition of the normal and selected strains. Some previous analyses of the haemolym...

Protease activities in the haemolymph and fat body in a bloodsucking insect, Rhodnius prolixus, infected with Trypanosoma rangeli, were investigated. After SDS-polyacrylamide gel electrophoresis containing gelatin as substrate, analysis of zymograms performed on samples of different tissues of controls and insects inoculated or orally infected with short or long epimastigotes of T. rangeli, dem...

Lectins play important role in innate immunity of animals. The affinity of the natural haemagglutinin of the giant freshwater prawn Macrobrachium rosenbergii towards vertebrate erythrocytes and its level with relation to sex, size and moult stages were studied. The strongest agglutinating titres in haemolymph of prawns were marked against guinea pig, chicken, Clarias batrachus, and rabbit eryth...