background: echinococcosis is a zoonotic parasitic disease of humans and various herbivorous domestic animals transmitted by the contact with domestic and wild carnivores,mainly dogs and foxes. the aim of this study is the production, purification and evaluation immunogenicity of new construction of eg95 protein. methods: the recombinant plasmid pet32-a+ used for eg95 expression was constructed...

background recommendations to immunize healthcare workers (hcws) against hepatitis b are well known. however, a proportion of individuals do not respond to the primary standard three-dose hb vaccination schedule. conclusions both the single- and double-dose hb vaccines were adequately immunogenic, and the double-dose hb vaccine was not significantly more immunogenic than the single-dose vaccine...

Recent interest in therapies for sickle cell anemia based on elevating fetal Hb has made accurate estimates of the sparing effect of fetal Hb (Hb F) and other non-sickle Hbs on sickle Hb (Hb S) polymerization essential. We have developed a technique, using HbCO as surrogate for HbO2, that enables us to assess the solubility of Hb S as a function of ligand saturation under conditions that mimic ...

BACKGROUND  Immune response to quadrivalent human papillomavirus (HPV) vaccine delivered at 0, 2, and 6 months in young adolescent females plateaus around 24 months after immunization. Antibody levels >24 months postvaccination using extended dosing schedules is unknown. METHODS  We conducted a follow-up immunogenicity study of adolescent girls in Vietnam who participated in a noninferiority ...

Hb Hoshida (␤43Glu3 Gln). All 4 patients with abnormal peaks at the S window on HPLC (mean retention time, 0.91 min) had Hb Queens (␣1 34Leu3 Arg). All 10 patients with variant peaks were heterozygous, and they had abnormal bands within the Hb S/Hb G/Hb D area on cellulose acetate Hb electrophoresis. Hb G Coushatta has been found in Koreans, Chinese, and in some Japa-nese families (6). This var...

BACKGROUND In sickle cell disease, the quantification of Hb A2 is important for the differential diagnosis between sickle cell anemia (Hb SS) and Hb S/β(0)-thalassemia. OBJECTIVE To determine Hb A2 levels as quantified by high performance liquid chromatography in patients with sickle cell anemia (Hb SS) and with the SC hemoglobinopathy, with or without concomitant alpha thalassemia. METHODS...

CASE DESCRIPTION A sample submitted for hemoglobin (Hb) evaluation on a 5-day-old premature male infant with intestinal perforation, intraventricular hemorrhage, anemia, and sepsis contained 61.1% Hb A, 23.7% Hb F, 2.1% Hb A2, and peaks of 8.2% and 5.2% in the P2 and P3 regions of the Bio-Rad Variant II HPLC thalassemia assay (Fig. 1). Isoelectric focusing showed Hb A, Hb F, Hb A2, and a band c...

A recombinant hemoglobin containing Val beta 7 (Hb beta E7V) was engineered and expressed in yeast to evaluate amino acid specificity of the Glu beta 6-->Val mutation (Hb beta E6V) in promoting polymer formation of deoxyhemoglobin. The purified CO Hb beta E7V migrated as a single band on electrophoresis with a slightly decreased positive charge compared with CO Hb S. The oxygen affinity of Hb b...