(Kdr), suggesting that they are hemangioblast precursors of the blood and vascular endothelial lineages. However, the preferential differentiation of PECAM positive vascular endothelial cells suggests that Pofut2 is essential for differentiation of the hematopoietic lineage. Many POFUT2 target proteins are constitutive components of ECM or associate with ECM. Among these, CCN, TSP, and ADAMTS f...

(Kdr), suggesting that they are hemangioblast precursors of the blood and vascular endothelial lineages. However, the preferential differentiation of PECAM positive vascular endothelial cells suggests that Pofut2 is essential for differentiation of the hematopoietic lineage. Many POFUT2 target proteins are constitutive components of ECM or associate with ECM. Among these, CCN, TSP, and ADAMTS f...

Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for transcripts encoding the G-protein-coupled APELIN receptor (APLNR). APLNR-positive cells, identified by binding of the fluoresceinated peptide ligand, APELIN (APLN), or an anti-APLNR mAb, were found in both posterior mesoderm and anterior mesendode...

A critical role for the endothelium of yolk sac and dorsal aorta has been shown in embryonic hematopoiesis. A stromal cell line derived from yolk sac, YSCL-72, has been chosen to search for a novel molecule associated with embryonic hematopoiesis. Analysis between YSCL-72 and an adult aorta-derived endothelial cell line, EOMA, demonstrated that activated leukocyte cell adhesion molecule (ALCAM,...

Hereditary thrombocythemia is a rare autosomal dominant disorder caused by mutations in either the thrombopoietin gene (TPO) or its receptor c-MPL. TPO mutations described so far lead to thrombopoietin overproduction through increased translation of m-RNA. Unilateral transverse reduction limb defects are usually sporadic and generally thought to be caused by vascular disruptions. Reports of inh...

The current progress using the human embryonic stem cell (hESC) model system has provided much insight into the early origins of the hematopoietic and endothelial lineages, particularly the elusive hemangioblast. Recently, the cellular hierarchy and molecular regulation controlling hematopoietic commitment have been further elucidated. These findings not only provide new insights into early hum...