The clinical presentation of alcoholic hepatitis (AH) can be mimicked by other alcoholic liver diseases. The aim of this study was to identify clinical features that predict AH on liver biopsy. Biopsies from patients hospitalized for presumed severe AH were used to identify a derivation cohort (101 patients) and validation cohort (71 patients). Using histologic scores for hepatocyte ballooning,...

The study evaluated the stability of an oligopeptide (KRQKYD) and its transport mechanism by simulating gastrointestinal digestion a model human intestinal Caco-2 monolayer cells in vitro. In this study, effects environmental factors (temperature, pH NaCl concentration) simulated on KRQKYD were indicators levels alanine transaminase (ALT), aspartate (AST) malondialdehyde (MDA) alcohol-induced h...

Background: Diffuse parenchymal liver diseases are contributing substantially to global morbidity and represent major causes of deaths worldwide. The aim our study is assess whether established hepatic fat iron quantitation relaxometry-based quantification hepatocyte-specific contrast material as surrogate for function estimation allows evaluate fibrosis.

Embryonic stem cell-derived hepatocyte precursor cells represent a promising model for clinical transplantations to diseased livers, as well as for establishment of in vitro systems for drug metabolism and toxicology investigations. This study aimed to establish an in vitro culture system for scalable generation of hepatic progenitor cells. We used stable transgenic clones of murine embryonic s...

I solve a conjecture originally studied by M. Ajtai. It states that for different primes q, p the matching principles Count(q) and Count(p) are logically independent. I prove that this indeed is the case. Actually I show that Count(q) implies Count(p) exactly when each prime factor in p also is a factor in q. 1 The logic of elementary counting “She loves me, she loves me not, she loves me,. . ....

Hepatocyte apoptosis and energy metabolism in mitochondria have an important role in the mechanism of acute liver failure (ALF). However, data on the association between apoptosis and the energy metabolism of hepatocytes are lacking. The current study assessed the activity of several key enzymes in mitochondria during ALF, including citrate synthase (CS), carnitine palmitoyltransferase‑1 (CPT‑1...

BACKGROUND Chronic Hepatitis B virus (HBV) infection can lead to the development of chronic hepatitis, cirrhosis and hepatocellular carcinoma. We hypothesized that HBV might accelerate hepatocyte ageing and investigated the effect of HBV on hepatocyte cell cycle state and biological age. We also investigated the relation between inflammation, fibrosis and cell cycle phase. METHODS Liver sampl...

Mouse embryonic stem cells (mESC) have been used to study lineage specification in vitro, including towards a hepatocyte-like fate, and such investigations guided lineage differentiation protocols for human (h)ESC. We recently described a four-step protocol to induce hepatocyte-like cells from hESC which also induced hepatocyte-like cell differentiation of mouse induced pluripotent stem cells. ...