High-performance liquid chromatography (HPLC) is a refinement of traditional column chromatographic techniques. The speed of analysis and the resolution are increased with new column-packing materials and eluant pumped through the column at high pressure. The potential for achieving measurements of hormones in small volumes of plasma or urine is limited, both in normal and pathological situatio...

The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...

A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.

Denaturing high-performance liquid chromatography (DHPLC) compares two or more chromosomes as a mixture of denatured and reannealed PCR amplicons, revealing the presence of a mutation by the differential retention of homo- and heteroduplex DNA on reversed-phase chromatography supports under partial denaturation. Temperature determines sensitivity, and its optimum can be predicted by computation...

We describe a "high-performance" liquid-chromatographic procedure for determination of creatinine in serum. Creatinine is separated from other species by cation-exchange chromatography. Absorbance of the creatinine in the eluate is monitored at 234 nm. Results from this procedure on sera correspond closely to those of a manual Jaffé-reaction procedure involving use of a protein-free filtrate wi...

mass spectrometry method is developed and validated for the simultaneous quantitation of three major phenolic acids including 1,5-dicaffeoylquinic acid (1,5-DCQA), 3,4-dicaffeoylquinic acid (3,4DCQA), and 3,5-dicaffeoylquinic acid (3,5-DCQA) in rat plasma. All analytes and internal standard (bergeninum) are extracted from plasma samples by liquid–liquid extraction with isopropanol. The chromato...

background and objectives: a method was developed and validated for determining 15 polycyclic aromatic hydrocarbons (pahs) in refined olive and refined pomace olive oils using high performance liquid chromatography coupled with a fluorescence detector. materials and methods: the sample preparation involved liquid–liquid extraction with organic solvent and low temperature clean-up. the low tempe...

the high mobility group (hmg) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (hplc). reversed-phase hplc under conditions of 50 mm triethylamine adjusted to ph 2.2 with phosphoric acid (solvent a) and 95% acetonitrile in water (solvent b) was used to separate proteins primarily on the basis of differences in the overall hydrophobicity....