Proteomics can be defined as the systematic analysis of proteins for their identity, quantity and function. In contrast to a cell's static genome, the proteome is both complex and dynamic. Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS). However, this technique is under scrutiny because of a failure to de...

Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethy...

pressure (Pe) and temperature (T ). Three-dimensional network shown in two perspectives. Conditions: 25 cm 4.6 mm i.d. packed column, 10 m silica particles; n-pentane. (Reprinted with permission from Lee ML and Markides KE (eds) (1990) Analytical Supercritical Fluid and Extraction. Provo, UT, USA: Chromatography Conferences, Inc.) Jusforgues P (1995) In: Subramanian G (eds) Process Scale Liquid...

A two-dimensional liquid chromatographic system is described here which uses size-exclusion liquid chromatography (SEC) followed by reversed-phase liquid chromatography (RPLC) to separate the mixture of proteins resulting from the lysis of Escherichia coli cells and to isolate the proteins that they produce. The size-exclusion chromatography can be conducted under either denaturing or nondenatu...

The convenience of dried blood filter paper specimens for genetic screening programs has prompted us to test the stability of these specimens for hemoglobin identification by cation exchange high performance liquid chromatography. This report shows that identification of Hb AA, Hb AF, Hb AS, Hb FAS, Hb AJ, Hb FJ, Hb EF, and Hb SS can be achieved by high performance liquid chromatography even af...

Lipids are important components in all biological tissues having many essential roles associated with the proper function of the organism. Their analysis in the biological tissues and body fluids is a challenging task due to the extreme sample complexity of polar lipids and to their amphiphilic character. In this work, we describe a new method for the characterization of the lipid composition i...

Automated two-dimensional liquid chromatography using the PF2D system from Beckman Coulter provides a fractionation platform well suited for differential proteomic studies. To date, the reliability and reproducibility of PF2D has not been accurately tested. Here, we used an optimized software and a pressure-resistant pH electrode, allowing a precise and reproducible control of the pH limits for...