BACKGROUND Unambiguous HLA typing is important in hematopoietic stem cell transplantation (HSCT), HLA disease association studies, and solid organ transplantation. However, current molecular typing methods only interrogate the antigen recognition site (ARS) of HLA genes, resulting in many cis-trans ambiguities that require additional typing methods to resolve. Here we report high-resolution HLA...

To investigate the human leukocyte antigen (HLA) association with anti-synthetase syndrome (ASSD). We conducted largest immunogenetic HLA-DRB1 and HLA-B study to date in a homogeneous cohort of 168 Caucasian patients ASSD 486 ethnically matched healthy controls by sequencing-based-typing. A statistically significant increase HLA-DRB1*03:01 HLA-B*08:01 alleles compared was disclosed (26.2% versu...

To assess possible associations between human leucocyte antigens (HLA) and the achievement of remission during gold treatment, HLA typing was performed in 67 rheumatoid arthritis (RA) patients with a gold-induced remission and in 25 control RA patients who discontinued gold therapy because of lack of efficacy. Both groups of RA patients showed a significantly higher frequency of DR4 antigen and...

It has been more than ten years since the first Human Leukocyte Antigen (HLA) matching for Fanconi anemia was reported, allowing successful haemopoietic reconstitution in affected sibling by transplantation of stem cells obtained from HLA-matched offspring (Verlinsky et al., 2000; Verlinsky et al., 2001). Preimplantation Genetic Diagnosis (PGD) in combination with HLA matching is being used to ...

AIM The purpose of this study was to investigate 50 women from eight families with familial cervical cancer in Wufeng County, Hubei Province, China, a region with a high incidence of cervical cancer. Eighty-nine healthy women, of similar age, location and ethnicity, were selected as a control group. METHODS Blood samples were collected from both groups, and HLA-A, HLA-B, and HLA-DRB1 genotype...

The human leucocyte antigen (HLA) has been documented to be involved in various disease susceptibilities or in resistance against certain diseases. An important element in susceptibility and resistance to disease is ethnic genetic constitution. Cognizant of this, the present study aimed at studying the prevalence of particular HLA class II in a normal healthy Malay population which may serve as...

In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a smal...