A commentary on Invasion of shoot apical meristems by Chrysanthemum stunt viroid differs among Argyranthemum cultivars Given that they do not encode proteins, viroid RNAs are extremely dependent on host factors for completing their infection cycle. This cycle consists of: (i) access to the organelle wherein they are replicated, the nucleus in the family Pospiviroidae (type member potato spindle...

Background and Aims: Viroids are smallest known plant pathogens and cause several economically significant diseases. Until recently, viroid detection relied mainly on biological tests and indexing. Today various diagnostic techniques such as nucleic acid hybridization, southern blot and reverse transcription coupled with polymerase chain reaction (RT-PCR) are being used for detection and diagno...

Over the past years deep sequencing experiments have opened novel doors to reconstruct viral populations in a high-throughput and cost-effective manner. Currently a substantial number of studies have been performed which employ next generation sequencing techniques to either analyze known viruses by means of a reference-guided approach or to discover novel viruses using a de novo-based strategy...

The c o m p l e t e n u c l e o t i d e s e q u e n c e of a viro i d i s o l a t e d from c i t r u s ( C i t r u s m e d i c a L. U S D C S 6 0 1 3 ) , d e s i g n a t e d as ci t r u s isolate of hop s t u n t v i r o i d ( H S V d t ) ( 1 ) , was e s t a b l i s h e d . T he sin g l e s t r a n d e d c i r c u l a r m o l e c u l e s of c i t r u s v i r o i d RNA c o n s i s t s of 3 02 n ...