Changes of glycosphingolipids (GSLs) in the bipotential cell differentiation of human promyelocytic leukemia cell line HL-60 cells were investigated by high-performance thinlayer chromatography (HPTLC). with special reference to morphological and functional changes, such as phagocytosis and nitroblue tetrazolium (NBT) reduction. Nine molecular species of neutral GSLs and 1 3 or more species of ...

A simple, precise and accurate high-performance thin-layer chromatographic method has been established for quantitative determination of quinine. Conditions were also optimized for best possible extraction of quinine via varying concentrations of diethyl amine in different solvents (n-hexane, chloroform, ethyl acetate and methanol) for maximum recovery of quinine. Methanol modified with 20 % DE...

Um método foi desenvolvido e validado para a determinação de ocratoxina A (OTA) em vinho. Foi utilizado um sistema contendo um detector de carga acoplada (CCD) para adquirir as imagens fluorescentes da micotoxina obtidas a partir das placas de cromatografia em camada delgada de alta eficiência (HPTLC) sob luz ultravioleta. O método desenvolvido mostrou recuperação média de 90,4%. O limite de qu...

The present study was aimed at identification, isolation and quantification of marker compound Lupeol in Derris heyneana (Wight and Arn) Benth. by using HPTLC. A simple and sensitive High-Performance Thin-Layer Chromatographic (HPTLC) method validation was carried out as per the ICH guidelines for the identification and quantification of biomarker lupeol in root, stem and leaves of Derris heyne...

Crataeva tapia Linn. ssp. odora (Jacob.) Almeida, a traditional medicinal plant, which is valued for its benefits in the management of urinary and inflammatory disorders. Leaves are externally rubefacient and used in rheumatism; internally they are given as febrifuge and tonic. The leaves also act as anti-periodic. The current study was therefore carried out to provide requisite pharmacognostic...

The effects of non-ionic surfactants as mobile phase additives on the fluorescence intensity of dansyl derivatives of biogenic amines in high-performance thin-layer chromatography (HPTLC) were investigated. The dansyl derivatives of putrescine, cadaverine, spermidine and spermine were separated by HPTLC with silica gel as stationary phase and an eluent consisting of chloroform–triethylamine (2 ...

Cotinine, as the main metabolite of nicotine, has been determined in urine using solid-phase extraction and the high-performance thin-layer chromatographic (SPE-HPTLC) method. The urine samples were collected from a group of 35 male adolescents which were moderate or significantly exposed to home environmental tobacco smoke (ETS). l-methyl-2-pyrrolidinone was used as the internal standard in th...

The present study investigates a quantitative high performance thin layer chromatographic (HPTLC) method for chlorogenic acid and hyperoside determination from berry extracts. Chlorogenic acid and hyperoside were extracted using a methanol and water mixture and separated on a HPTLC 60F254 silica gel plates with ethyl acetate, acetic acid, formic acid and water 10:1.1:1.1:2.3 (v/v/v/v) as mobile...

A simple, precise and sensitive high performance thin layer chromatographic (HPTLC) method has been developed and validated for drug of choice Lumefantrine in treatment of malaria (P. falciparum). Silica gel 60 F254 HPTLC precoated plates were used for quantitative analytical purpose. Methanol water 9.5 + 0.5 (v/v) was used as the solvent system. Densitometric scanning was carried out with deut...

A sensitive and accurate high-performance thin layer chromatography (HPTLC) method has been developed to determine the quantity of 6-gingerol in the methanolic extract of Zingiber officinale containing dietary supplements, teas and commercial creams. 6-gingerol was separated on aluminumbacked silica gel 60 F254 plates with n-hexane:ethyl acetate 40:60 (%, v/v) as mobile phase. A compact band wa...