Over the years a massive amount of high-throughput screening (HTS) data has been collected, however, the data are mainly utilized for providing lead generation programs with chemical entry points. The comparison of molecular structures and HTS data across many projects allows to identify and validate structural patterns of frequent hitters, i.e. compounds which generate multiple hits in various...

High-throughput sequencing (HTS) of antibody repertoire libraries has become a powerful tool in the field of systems immunology. However, numerous sources of bias in HTS workflows may affect the obtained antibody repertoire data. A crucial step in antibody library preparation is the addition of short platform-specific nucleotide adapter sequences. As of yet, the impact of the method of adapter ...

BACKGROUND Intracranial hypertension, defined as an intracranial pressure (ICP) >20 mmHg for a period of more than 5 min, worsens neurologic outcome in traumatic brain injury (TBI). While several mechanisms contribute to poor outcome, impaired cerebral perfusion appears to be a highly significant common denominator. Management guidelines from the Brain Trauma Foundation recommend measuring ICP ...

OBJECT Currently, mannitol is the recommended first choice for a hyperosmolar agent for use in patients with elevated intracranial pressure (ICP). Some authors have argued that hypertonic saline (HTS) might be a more effective agent; however, there is no consensus as to appropriate indications for use, the best concentration, and the best method of delivery. To answer these questions better, th...

Most studies on Mandarin HTS (HMM-based text-to-speech system) have taken the initial/final as the basic acoustic units. It is, however, challenging to develop a multilingual HTS in a uniformed and consistent way since most of other languages use the phoneme as the basic phonetic unit. It becomes hard to apply cross-lingual adaptation which need map phonemes from each other, particularly in the...

BACKGROUND Hyperosmolar solutions have been used in neurosurgery to modify brain bulk and prevent neurological deterioration. The aim of this animal study was to compare the short-term effects of equivolemic, equiosmolar solutions of mannitol and hypertonic saline (HTS) on cerebral cortical microcirculation in a rabbit craniotomy model. METHODS Rabbits (weight, 2.0-3.0 kg) were anesthetized, ...

photoproteins are excellent reporter systems because they don’t have virtually background signal. aequorin is the most well-known photoprotein. three improved engineered photoproteins photina, i-photina and c-photina, were also recently developed and optimized for generation of ca2+ mobilization assays precisely. the total light emission is greater than aequorin and their reaction kinetics is a...

In cytomics bookkeeping of the data generated during lab experiments is crucial. The current approach in cytomics is to conduct High-Throughput Screening (HTS) experiments so that cells can be tested under many different experimental conditions. Given the large amount of different conditions and the readout of the conditions through images, it is clear that the HTS approach requires a proper da...

We have analysed retrospectively the clinical features and electroencephalograms in 35 patients with complex partial seizures of temporal lobe origin who were seizure-free after epilepsy surgery. Two groups were differentiated for statistical analysis: 16 patients had hippocampal temporal lobe seizures (HTS) and 19 patients had extrahippocampal temporal lobe seizures (ETS) associated with a sma...

Objective: Hormone Therapy (HT) is usually introduced to patients with hormone-receptor-positive metastatic breast cancer without life-threatening metastasis prior to chemotherapy. Many physicians expect HT not to affect the efficacy of subsequent chemotherapy, but there is no evidence to confirm this. In this retrospective study, we investigated the efficacy of chemotherapy after hormone thera...