Immunochemical and functional characteristics of tumor cell membrane proteins and human platelet glycoproteins were studied. Immunoblotting revealed that membrane proteins of a cultured breast tumor cell line (BT-20) had three protein bands, which were each recognized by monoclonal antibodies to human platelet glycoprotein Ib, IIb, and IIIa, suggesting some immunochemical similarities between t...

Primary immune thrombocytopenic purpura (ITP) is an acquired immune-mediated disorder characterized by transient or persistent decreased platelet count (<100 × 109/L) that affects children and adults in the absence of other underlying causes.1,2 The low platelet count results from platelet destruction by antiplatelet autoantibodies associated to causes such as insufficient platelet production, ...

Washed human platelets were labeled with 1251 by the lactoperoxidase-catalyzed method and solubilized in 1 % Triton X-100. The soluble proteins were analyzed by crossed-immunoelectrophoresis in 1 % agarose. employing a rabbit antibody raised against whole human platelets. Analysis of autoradiograms developed from dried agarose gels led to the establishment of a normal reference pattern that was...

Fetal/neonatal alloimmune thrombocytopenia (FNAIT) is often caused by maternal alloantibodies against the human platelet antigen (HPA)-1a, which opsonizes fetal platelets (PLTs). Subsequent PLT destruction is mediated via the Fc part of the alloantibodies. The monoclonal antibody (mAb) SZ21 binds to the HPA-1a epitope and inhibits the binding of maternal alloantibodies. However, it also promote...

This report describes a new low-frequency alloantigen, Oea, responsible for a case of neonatal alloimmune thrombocytopenia (NAIT). In a population study none of 600 unrelated blood donors was an Oea carrier. By immunochemical studies the Oea antigen could be assigned to platelet glycoprotein (GP) IIIa. Sequencing of GPIIIa complementary DNA from an Oea (1) individual showed deletion of a lysine...

Platelets are pivotal to the process of arterial thrombosis resulting in ischemic stroke. Occlusive thrombosis is initiated by the interaction of von Willebrand factor (vWf) and platelet glycoprotein (GP) Ibalpha. Three polymorphisms have been described in GP Ibalpha (Kozak T/C polymorphism, variable number of tandem repeats [VNTR], and the human platelet antigen 2a [HPA-2a] [Thr] or HPA-2b [Me...

There is now an expanding volume of evidence to support the view that commensal organisms within the gut play a role in early programming and later responsivity of the stress system. The gut is inhabited by 10¹³-10¹⁴ micro-organisms, which is ten times the number of cells in the human body and contains 150 times as many genes as our genome. It has long been recognised that gut pathogens such as...

A N UNDERSTANDING of the structure of human platelet membrane antigens is of both biologic’ and clinical7 interest. HLA-A,B antigens’ and those of the ABH system2 are present on platelets. However, adsorption of exogenous soluble HLA-A,B antigens34 and ABH substances5 onto platelets has been demonstrated in vitro. This raises the question of whether these antigens are integral membrane proteins...

Abstract Sepsis and autoimmune diseases remain major causes of morbidity mortality. The last decade has seen a new appreciation platelets in host defense, both immunity thrombosis. Platelets are first responders the blood to microbes or non?microbial antigens. role physiologic is counterbalanced by their pathology, for example, microvascular encounter antigens via innate adaptive immun...