A trial was made of hyaluronidase and potassium chloride as adjuvants for epidural analgesia in 137 patients. Lignocaine hydrochloride in 2 per cent concentration with adrenaline 1/200,000 was used as the control solution in 61 cases; hyaluronidase, 5 USP units/ml, was added in 26 cases, and 1 per cent potassium chloride was added in 50 cases. Hyaluronidase tended to impair the quality of senso...

Over the past 60 years, hyaluronidase has been successfully utilized in ophthalmic surgery and is now being implemented in dermatosurgery as well as in other surgical disciplines. The enzyme is considered a "spreading factor" as it decomplexes hyaluronic acid (also called hyaluronan, HA), an essential component of the extracellular matrix (ECM). When applied as an adjuvant, hyaluronidase enhanc...

The most recently published method for the assay of testicular hyaluronidase preparations was based on the premise that the enzyme also exhibited carboxylesterase activity towards indoxyl acetate. Studies on the relative enzyme activities of various hyaluronidase preparations towards hyaluronate and indoxyl acetate, the relative stabilities towards pH, temperature and mechanical shaking and the...

Hyaluronan is a major component of the extracellular matrix and glycocalyx. Its main somatic degrading enzymes are hyaluronidases 1 and 2, neither of which is active in the bloodstream. We generated hyaluronidase 2-deficient mice. These animals suffer from chronic, mild anemia and thrombocytopenia, in parallel with a 10-fold increase in plasma hyaluronan concentration. In this study we explored...

OBJECTIVE To analyze the effects of hyaluronidase and hirudoid treatment on drug extravasation in neonates. METHODS The medical records of 13 neonates with drug extravasation treated with hyaluronidase and hirudoid between August 1(st), 2010 and May 1(st), 2012 were analyzed retrospectively. The treatment procedure for drug extravasation adhered to the protocol in neonatal department. The inf...

BACKGROUND Hyaluronic acid (HA) preparations are the most commonly used injectable fillers in esthetic medicine. In contrast to other injectable fillers with hyaluronidase, there is a tool available to reduce unwanted depots of this filler. AIMS The aim of this paper is to give an overview on the present literature and in addition to give some recommendations for use based on the experience o...

OBJECTIVE To investigate whether hyaluronidase (Hyalase) is a useful and justified addition to haematoma block for pain relief. METHODS The study was a randomised double blind trial of 33 consecutive patients attending the accident and emergency department for manipulation of distal radius fracture under haematoma block. Control patients received 1% lignocaine; the treatment group received 1%...

Jaagsiekte sheep retrovirus (JSRV) uses hyaluronidase 2 (Hyal2) as a cell entry receptor. By making inactivating mutations to the catalytic residues of human Hyal2, we found that hyaluronidase activity was dispensable for its receptor function. The affinities of the JSRV envelope glycoprotein for Hyal2 and the Hyal2 mutant were similar, and hyaluronan did not block either high-affinity interact...

1. It has been shown that a number of proteolytic enzymes and snake venom, in relatively small amounts, and within a wide range of pH variation, will restore hyaluronidase activity after its inhibition by serum. 2. The known properties of the venom protease are found to be identical with those of Haas' "proinvasin I." It is concluded that the protease of the venom offers adequate explanation fo...

'l'hc mechanisms responsible for the invasivencss of malignant tumors remain largely unknown. One explanation proposed is that malignant tumors may form a spreading factor that breaks down the barriers in surrounding normal tissues and thus permits penetration of tumor cells (6). If it exists, such a spreading factor would probably be hyaluronidasc which hydrolyses the connective tissue constit...