Safferman, Robert S. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), and Mary-Ellen Morris. Growth characteristics of the blue-green algal virus LPP- 1. J. Bacteriol. 88:771-775. 1964.-The blue-green algal (BGA) virus, strain LPP-1, formed two distinct plaque variants. During subsequent propagation, each of the isolated variants eventually reverted to a mixture of both plaque ty...

Following a study of the spectrophotometric properties of ortho-tolidine and its oxidation products, with particular attention to variation brought about by change in the hydrogen ion concentration, a method for the quantitative estimation of haemoglobin in serum and urine in which ortho-tolidine is substituted for benzidine in a peroxidase system is described. The method is designed to measure...

In a previous paper (1) the inactivation of catalase by heat at different pH was studied. It was discovered that the heat inactivation depends upon the hydrogen ion concentration of the enzyme solution which affects both the velocity and the extent of the reaction. It was also found there that 65” was the critical temperature for the enzyme preparation since at this temperature the catalase was...

A technic is described for collecting arterialized capillary blood in a small heparinized polyethylenecentrifuge tube. The pH is measuredafter dilution and injection into a BeckmanmicrobloodpH Assembly. Dilution of the blood with saline results in an averageincreaseof 0.027 pH units. This changein pH on dilution is eliminated by inclusionof calcium in the diluent. The pH of capillary blood draw...

A study of the effect of acetamide as an accelerator of the reaction between free bilirubin and diazotized sulfanilic acid (DSA) has led to its use in a method for serum bilirubin which is simple, sensitive, linear over a wide range, and which requires neither excessive dilution nor protein precipitation. The conditions necessary for greatest sensitivity and for linearity have been established ...

1. The human gall bladder acidifies the bile. In this respect its action is similar to that of the gall bladders of lower animals, previously described by other workers. 2. The hydrogen ion concentration of gall bladder bile is increased considerably in cases of obstruction of the common or cystic ducts. The highest values were found following complete obstruction. 3. The occurrence of gall sto...

1. In the growth and death of the pneumococcus in fluid media containing 1 per cent glucose the production of acid is the most important bactericidal factor. 2. 1 per cent glucose bouillon cultures of the pneumococcus allowed to grow and die out usually reach a final acidity of a pH of about 5.1. 3. At a hydrogen ion concentration of about 5.1 or higher, the pneumococcus does not survive longer...

The mechanism of acidification by the urinary bladder of the water turtle was studied in an in vitro system which permitted control and measurement of electrical and concentration driving forces. The rate of hydrogen ion secretion was measured by means of a pH stat technique in the absence of exogenous carbon dioxide and bicarbonate. Transport of hydrogen ion into the solution bathing the mucos...

1. Under the conditions of these experiments, there appears to be a distinct and constant difference in the final hydrogen ion concentration of Streptococcus haemolyticus from human and bovine sources. 2. Of 124 strains of Streptococcus haemolyticus from known human origin, 116 reached a final hydrogen ion concentration of from pH 5.0 to 5.3. Only 8 reached a pH more acid than 5.0 and none more...

1. The ability of marine fishes to absorb oxygen at low tension from the sea water is more or less dependent upon the hydrogen ion concentration of the water. 2. The ability of fishes to withstand wide variations in the range of hydrogen ion concentration of the sea water can be correlated with their habitats. The fishes that are most resistant to a wide variation in the hydrogen ion concentrat...