BACKGROUND β-lactoglobulin hydrolysates (BLGH) have shown antioxidant, antihypertensive, antimicrobial, and opioid activity. In the current study, an innovative combination of high hydrostatic pressure and enzymatic hydrolysis (HHP-EH) was used to increase the yield of short bioactive peptides, and evaluate the anti-inflammatory and antioxidant properties of the BLGH produced by the HHP-EH proc...

The diagnosis and treatment of cow's milk protein allergy (CMPA) is still a challenge. A systematic literature search was performed using Embase, Medline, The Cochrane Database of Systematic Reviews and Cochrane Central Register of Controlled Clinical Trials for the diagnosis and treatment of cow's milk allergy (CMA). Since none of the symptoms of CMPA is specific and since there is no sensitiv...

This study investigated the effects of protease treatments (trypsin, chymotrypsin, and pepsin) under various pressure levels (0.1-300 MPa) for the characteristics of porcine placenta hydrolysates. According to gel electrophoretic patterns, the trypsin showed the best placental hydrolyzing activity followed by chymotrypsin, regardless of the pressure levels. In particular, the peptide bands of t...

Casein and whey proteins with and without heat treatment were obtained of whole milk and four commercial milks in Ecuador, and were hydrolyzed. Then, their capacity to inhibit the lipid peroxidation using the TBARS method was evaluated at concentrations of 0.02, 0.04, 0.2, and, 0.4 mg/mL. Native and heated hydrolysates of milk proteins present high inhibitions of lipid peroxidation with a dose ...

1. Partial hydrolysates of phytic acid, containing mixtures of different polyphosphate esters of inositol, have been shown to be potent inhibitors of the calcification in vitro of ricketic rat cartilage and rat aorta; phytate itself was inert. 2. Phytic acid, as well as its hydrolysates, when injected parenterally, prevented aortic calcification in rats with vitamin D intoxication. 3. The rat a...

that whilst exchange may play only a small part in normal protein synthesis in vivo, it may become a dominant route for amino acid incorporation in vitro when cellular organization is disrupted or modified. 3. The radioactivity in crude enzymic hydrolysates of yeast or Chlorelta protein is much more rapidly incorporated into ribonuclease than is the activity of purified hydrolysates. The activa...

DNA gyrase inhibitors, cyclothialidines B, C, D and E were isolated from four Streptomycete strains (NR 0659, NR 0660, NR 0661 and NR 0662). Their structures have been elucidated based on the amino acid analysis of the hydrolysates, NMR and HRFAB-MS experiments and shown to be cyclothialidine analogs. The absolute stereochemistry has been determined by the chiral HPLC analysis of the hydrolysat...

BACKGROUND Casein is the main proteinaceous component of milk and has made us interest due to its wide applications in the food, drug, and cosmetic industries as well as to its importance as an investigation material for elucidating essential questions regarding the protein chemistry. Enzymatic hydrolysis is an important method commonly used in the modification of protein structure in order to ...

The history of amino acid assay has been marked by numerous controversies on the problem of destruction of amino acids by acid hydrolysis of the protein or food. It has been generally conceded that when such hydrolyses are carried out in the presence of certain carbohydrates amino acids are destroyed. Recently Baldwin, Lowry, and Thiessen (1) reported that autoclaving casein with dextrose resul...

Cow's milk whey consists of many protein components and some of them are antigens to human and known to modulate immune responses. Enzymatic hydrolysis is a useful method to modify proteins with allergenicity. The objective of this study was to identify whether the in vitro enzymatic hydrolysis could reduce the allergenicity of whey protein concentrates (WPC). In this study, WPC were hydrolyzed...