Hydroxy-L-proline was first isolated by Fischer in 1902 from a hydrolysate of gelatin (l).l The synthesis and determination of the structure were reported by Leuchs and coworkers (5--g), and, in 1919, the separation of the two racemic stereoisomers and the resolution of each racemate through mixtures of quinine with the corresponding N-phenylisocyanate derivatives were accomplished (5). In the ...

A modified assay for the determination of hydroxyproline in tissue is presented. The modifications greatly reduce the time required for analysis of excised tissue as first introduced by Stegemann and Stalder [1]. These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. The analysis utilizes the standard ad...

Nghiên cứu được tiến hành nhằm đánh giá tác dụng chống xơ gan của sản phẩm Vismisco ở các mức liều 0,6 g/kg/ngày và 1,8 trên mô hình thực nghiệm gây bằng tiêm màng bụng dung dịch CCl4 liên tục trong 19 tuần chuột nhắt trắng chủng Swiss. Các chỉ số bao gồm trọng lượng tương đối, hoạt độ enzym gan, nồng MDA hydroxyproline ảnh vi thể. Kết quả nghiên cho thấy đã thể hiện hạn chế tổn thương hóa do r...

I . Hydroxyproline was determined in urine, carcass, and skin of young rats fed on lowprotein and low-calorie diets. 2. Protein and calorie deprivation in young rats resulted in a marked fall in the soluble as well as insoluble collagen content of the skin. 3. The collagen content of the carcass (excluding skin) was slightly greater in the deficient rats than in the weanling rats, but very much...

Serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) is largely located in mitochondria in carnivores, whereas it is entirely found within peroxisomes in herbivores and humans. In rat liver, SPT/AGT is found in both of these organelles, and only the mitochondrial enzyme is markedly induced by glucagon. Although SPT/AGT is a bifunctional enzyme involved in the metabolism of both L-serin...

et al., 1967; Zorab et al., 1970). Allison, Walker, and Smith (1966) showed that the ratio of total hydroxyproline to creatinine in a 24-hour collection of urine gives more consistent results than measurement of hydroxyproline alone, an observation which has been extended to random samples of urine collected during waking hours (Howells, Wharton, and McCance, 1967; Younoszai et al., 1969). Whar...

Collagen turnover was studied in mouse fibroblast cultures (3T6) by radioactive labelling and compartmental analyses. The incorporation of [C]proline into protein during continuous labelling rapidly reached a maximum value which was directly proportional to the medium specific activity. Radioactivity appeared more slowly in hydroxyproline, and gradually accumulated as cultures became enriched i...

Free hydroxyproline inhibits the growth of animals1' I and plants.3-7 The mechanism of this inhibition is not known. Among the theoretically possible effects of hydroxyproline are: (1) reduction of protein synthesis by lowering proline incorporation, (2) interference with proline hydroxylation, and (3) direct incorporation of hydroxyproline resulting in nonfunctional proteins. Hydroxyproline re...