Ribonuclease-resistant infectious double-stranded RNA has been isolated in milligram quantities from poliovirusinfected HeLa cells. Purification was accomplished by eliminating high molecular weight single-stranded RNA by differential NaCl precipitation and chromatographing the remaining RNA on columns of methylated albumin-Kieselguhr. The product has the following physicochemical and biologica...

Cultures on autoclaved maize of the phytopathogenic fungus, Drechslera campanulata, were incorporated into diets and fed to male inbred BDIX rats. In a pilot trial, a diet containing 30% D. campanulata culture material killed 5 out of 5 rats in 15-25 days. Lesions included gastric corpus erosions, gastrorrhagia and ulcerative typhlitis. Diets containing 5% or 10% culture material induced erosiv...

Propolis is a resinous substance collected from plants by bees. Its composition depends on the vegetation, the season, and the source area. It usually contains many chemical compounds such as polyphenols, steroids and amino acids. The hereditary spherocytosis (HS) is a type of anaemia characterized by microcytic and hyperchromic red cells, spherical in shape and without central pallor. Clinical...

It has been shown that mice with complete deficiency of all 4.1R protein isoforms (4.1 / ) exhibit moderate hemolytic anemia, with abnormal erythrocyte morphology (spherocytosis) and decreased membrane stability. Here, we characterized the Gardos channel function in vitro and in vivo in erythrocytes of 4.1 / mice. Compared with wild-type, the Gardos channel of 4.1 / erythrocytes showed an incre...

The three accepted and well-defined types of familial haemolytic anaemia are familial spherocytic anaemia, sickle-cell anaemia, and Mediterranean anaemia. Reports have appeared of other types of familial anaemia, some of which are undoubtedly haemolytic. A family is here described in which four members in three generations were found to have non-spherocytic haemolytic anaemias. Case Histories C...

The hyperchromic effect has been used to detect the effect of tau on the transition of double-stranded DNA to single-stranded DNA. It was shown that tau increased the melting temperature of calf thymus DNA from 67 to 81 degrees C and that of plasmid from 75 to 85 degrees C. Kinetically, rates of increase in absorbance at 260 nm of DNA incubated with tau were markedly slower than those of DNA an...

Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the interaction mechanism and design new drug delivery systems. Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity measurement, circular dichr...

Anemia is considered one of the most common diseases that affect human body. In case prolonged existence, it can lead to development chronic due excessive load on vital organs. Effective treatments are available with early detection disease and largely depend its underlying cause. terms severity, anemia be divided into mild, moderate, severe according level hemoglobin. The morphological classif...

Case presentation: Patient L.V.M.B., female, 3 months old, born and resident in Frutal-MG. She was admitted to the Pediatric Emergency Room of Hospital de Clínicas da UFTM, referred from city origin, due an unprecedented convulsive crisis 1 day ago, which characterized by spastic movements topography hemiface on right (right eye traction labial commissure) preceded hyporexia irritability, accor...