A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe d...

Raspberry ringspot virus (RpRSV), which has not occurred in Japan, infects many plants such as raspberry, gooseberry, cherry, grapevine, and strawberry. Biological assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcription polymerase chain reaction (RT-PCR) have been introduced for the detection of RpRSV in postentry quarantine in Japan. We studied reverse transcription loop-me...

This review presents the basic principles of application loop-mediated isothermal amplification (LAMP) reaction for rapid diagnosis coronavirus infection caused by SARS-CoV-2. The technical details method, and most popular approaches specific non-specific detection products are briefly described. We also discuss first published works on use method nucleic acid SARS-CoV-2 virus, including those ...

A differential detection method for three wheat viruses: Wheat yellow mosaic virus (WYMV), Japanese soil-borne mosaic virus (JSBWMV) and Chinese wheat mosaic virus (CWMV) using reverse transcription loop-mediated isothermal amplification (RT-LAMP) reaction was developed. All three primer sets, which were designed from the genome sequences of WYMV, JSBWMV and CWMV respectively, worked most effic...

Novel rapid, sensitive, and simple detection assays that can be routinely used to prevent the transmission of Salmonella in the pork industry are in high-demand. Reverse-transcriptase Loop-mediated isothermal Amplification (RT-LAMP) assay is a novel molecular method that has advantages over real-time reversetranscriptase Polymerase Chain Reaction (RT-PCR) in that it does not require expensive e...

The Middle East respiratory syndrome coronavirus (MERS-CoV), an emerging human coronavirus, causes severe acute respiratory illness with a 35% mortality rate. In light of the recent surge in reported infections we have developed asymmetric five-primer reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for detection of MERS-CoV. Isothermal amplification assays will fac...

Due to the limitation of rapid development of specific antiviral drug or vaccine for novel emerging viruses, an accurate and rapid diagnosis is a key to manage the virus spread. We developed an efficient and rapid method with high specificity for the Middle East Respiratory Syndrome coronavirus (MERS-CoV), based on one-pot reverse transcription loop-mediated isothermal amplification (one-pot RT...

RT-LAMP法を用いて，ナシ（Pyrus spp.）の葉からナシ葉退緑斑点随伴ウイルス（PCLSaV）を検出する技術を開発した。設計したプライマーセットのうち，R2LP4が最も増幅が早く，退緑斑点症状のあるナシ葉の全RNAから15分以内にPCLSaVが検出された。12県で採取されたニホンナシおよびセイヨウナシの葉を用いた診断では，RT-LAMP法は既報のRT-PCR法と同等の感度と精度を示した。さらに，注射針で採取した汁液を用いた簡易なRT-LAMP検定においてもPCLSaVを検出することができた。また，塩基配列情報の比較から，設計したプライマーの一部は，日本以外で発生が確認されているPCLSaVの中国分離株と適合する可能性が示された。

We demonstrate an integrated microneedle (MN)-smartphone nucleic acid amplification platform for “sample-to-answer” diagnosis of multiplexed plant pathogens within 30 min. This portable system consists a polymeric MN patch rapid extraction minute and 3D-printed smartphone imaging device loop-mediated isothermal (LAMP) reaction detection. expanded the technology DNA targets as in previous study ...