viral infections are the cause of great economic losses in the poultry industry. development of appropriate reagents and serological diagnostic kits will help to control these infections. the aim of this study is to prepare a peroxidase labeled anti-chicken igg using a mab (5b8) against chicken igg, for detection of chicken antibodies in elisa. hybridoma cells producing the mab 5b8 were culture...

AIMS To investigate the diagnostic value of mucosal IgA and IgG Helicobacter pylori antibodies. METHODS The study population comprised 209 consecutive patients with severe dyspeptic complaints referred for upper gastrointestinal endoscopy. A positive culture or histological identification of H pylori in gastric biopsy specimens, or both, were used to confirm infection. Specific IgA and IgG H ...

Polyethylene glycol (PEG) is a biocompatible polymer that is often attached to therapeutic molecules to improve bioavailability and therapeutic efficacy. Although antibodies with specificity for PEG may compromise the safety and effectiveness of PEGylated medicines, the prevalence of pre-existing anti-PEG antibodies in healthy individuals is unclear. Chimeric human anti-PEG antibody standards w...

We studied the presence of immunoglobulin G (IgG) in equid fossil bones from Venta Micena (Granada, Spain), dated at 1·6 Myr old, and from Atapuerca (Burgos, Spain) (0·12 Myr), and in ostensibly hominid fossils VM1960 and VM3691 (1·6 Myr) from Venta Micena and CV1 and CV2 (1·4 Myr) from Cueva Victoria (Murcia, Spain). Samples taken from the specimens were crushed to a fine powder and their mine...

Rabbit immunoglobulin (Ig)M and IgG antibodies to Brucella abortus and Bordetella pertussis were isolated as purified products and their specific secondary biological activities were compared. IgM antibodies were found to be more active than IgG proteins in inducing agglutination and sensitization of B. abortus for the complement-dependent bactericidal effect and in inhibiting B. pertussis-indu...

Advanced glycation end-product (AGE)-damaged IgG occurs as a result of hyperglycemia and/or oxidative stress. Autoantibodies to IgG-AGE were previously demonstrated in patients with severe, longstanding rheumatoid arthritis (RA). We investigated whether IgG-AGE and anti-IgG-AGE antibodies were present early in the course of RA and other inflammatory arthropathies. We prospectively followed a co...

Serum antibodies to the stratum corneum of rat oesophagus epithelium, so-called 'antikeratin antibodies', have been largely demonstrated in rheumatoid arthritis (RA). IgM and IgG antibodies to this epithelium were studied by semiquantitative immunofluorescence in 528 patients with perfectly characterised rheumatic diseases, including 178 with classical or definite RA. Histological analysis of I...

A total of 52 serum samples from patients with symptoms suggestive of tick-borne encephalitis virus (TBEV) infection and positive IgM and/or IgG antibodies were tested for IgG avidity. Acute/recent TBEV infection was confirmed by low/borderline avidity index (AI) in 94.8% IgM positive/IgG positive samples, while in 5.2% high AI was found indicating persisting IgM antibodies. Majority of IgM neg...

OBJECTIVE Role of reactive oxygen species (ROS) modified human Immunoglobulin G (IgG) in systemic lupus erythematosus (SLE) has been investigated. METHODS Human IgG was modified by hydroxyl-radicals. Immunogenicity of native and modified human IgG was probed by inducing polyclonal antibodies in rabbits. Cross-reactions of induced antibodies with nucleic acid, chromatin, different blood protei...

A patient with atypical rheumatoid arthritis (RA) and local synovial synthesis of oligoclonal IgG in an arthritic knee joint is described. Measles virus-specific antibodies isolated from the synovial fluid (SF) were carried by oligoclonal IgG proteins but constituted only a fraction of the total oligoclonal IgG of the SF. Smooth muscle antibodies were markedly increased in the SF compared with ...