Objective(s): Resistance to medications is one of the main complications in chemotherapy of cancer. It has been shown that some multidrug resistant cancer cells indicate more sensitivity against cytotoxic effects of TNF-α compared to their parental cells. Our previous findings indicated vulnerability of the mitoxantrone-resistant breast cancer cells MCF-7/MX to cell de...

Western Blot tekniği, herhangi bir örnekten istenen tek proteini özgün şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu prot...

Electrophoresis fractionates nucleosomes which possess different protein compositions. We report here a procedure for transferring the DNA components of electrophoretically resolved nucleosomes to diazobenzyloxymethyl cellulose (DBM-paper). Histones are first removed from nucleosome components by electrophoresis in the presence of cetyltrimethylammonium bromide (CTAB), leaving DNA fragments fix...

BACKGROUND Trichinella spiralis is a zoonotic parasitic nematode that causes trichinellosis, a disease that has been identified on all continents except Antarctica. During chronic infection, T. spiralis larvae infect skeletal myofibres, severely disrupting their differentiation state. METHODOLOGY AND RESULTS An activity-based probe, HA-Ub-VME, was used to identify deubiquitinating enzyme (DUB...

The measurement of glycosylated haemoglobin in blood is vital for monitoring metabolic control in diabetics [1-3]. The development of many different methods of measurement (based on cation-exchange chromatography, electrophoresis, high performance liquid chromatography [HPLC], affinity chromatography, radio and colorimetric immuno techniques) proves the importance of this assay [4]. Since the f...

− The molecular imprinting technique is a reliable technique for the preparation of materials of predetermined selectivity. It is highly significant in research of the structure of enzyme, mechanism of receptor-antibody and analytical chemistry. In analytical separation science, molecularly imprinted polymers have been applied in several analytical techniques, such as liquid chromatography, cap...

Cellubrevin is one of the proteins involved in the docking and fusion of secretory granules to the plasma membrane. It has been reported that cellubrevin is widely distributed in both neural and non-neural cells, including insulin-secreting B-cells. This study aims to demonstrate by immunohistochemical techniques that cellubrevin is localized in insulin-secreting cells and further to examine wh...

The genes for acetylcholinesterase (ACHE) and butyrylcholinesterase (CHE) are expressed in multiple tumor tissues, including ovarian carcinomas. Both CHE and ACHE genes coamplify in leukemias. To examine the relationship of gene amplification to the expression of these genes in tumors, ACHE and CHE genes and their expression were studied in primary ovarian carcinomas. DNA blot hybridization dem...

Immunoglobulins (Ig) in feline sera and secretions were identified by immuno-electrophoresis and immunodiffusion with rabbit antisera prepared to feline IgG, IgA, IgM, and whole serum. Adult cat sera, colostral whey, postcolostral sera, tears, and nasal secretions contained IgG, IgA, and IgM. IgG was the only Ig identified in precolostral sera and cerebrospinal fluid. Milk, intestinal contents,...