It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...

Objective(s): The aim of this study was to prepare fraction and determine the biological activities of the polyphenol-enriched fraction of Berberis integerrima Bunge fruits. Materials and Methods: In this assay fraction was extracted by column chromatography, using Amberlite column as the stationary phase. Phenol and flavonoids in the extract and fraction were analyzed by high performance liqui...

There are as many measurement values of the true column hold-up volume, V0, techniques applied to evaluate this most important property in liquid chromatography (LC). The relative errors made on V0 measurements using conventional “non-retained” markers—such acetone, uracil, or thiourea reversed-phase (RPLC), benzene acenaphthene hydrophilic interaction (HILIC)—can be large ±30%. This situation ...

It has long been recognized that, with p-diazobenzenesulfonic acid (van den Bergh reagent), bilirubin in serum of patients with jaundice may exhibit two types of reaction (1, 2). If coupling with the diazo reagent occurs promptly in aqueous solution, the reaction is said to be “direct;” if, however, coupling t.akes place only after prior addition of alcohol, the reaction is said to be “indirect...

The availability of deuterium-labeled nicotinic acid makes stable isotope dilution mass spectromerty (MS) coupled with liquid chromatography (LC) an attractive option for the determination of the water-soluble B-vitamin niacin in food samples. A method was developed based on acid digestion, solid-phase extraction with a strong cation exchange column, and reversed-phase chromatography with a C18...

A method is described for separating antibody-bound from free fractions in ligand assays by column affinity chromatography, and its application to radioimmunoassay of choriomammotropin. In the method, 70 x 10 mm (i.d.) polypropylene columns containing about 150 mg of immunosorbent (goat anti-rabbit gamma-globulins covalently linked to Sepharose CL-4B) are used. Standards or unknowns, tracer and...

SUZUKI, M., OSAWA, S. and HIRANO, M. A Lyeium Chinense Miller Component Inducing Ovulation in Adult Female Rabbits. Tohoku J. exp. Med., 1972, 106 (3), 219-231Intravenous injection of the crude extract from Lycii folium among components of Lycium chinense Miller induced ovulation in the adult female rabbit. Chemical properties of the active component of this crude extract were investigated by d...

The reaction at room temperature of an aromatic aldehyde with pyrrole in the presence of Iodine, acetic acid catalyzed of meso-substituted dipyrromethane. The mixture was ground together in a mortar with a pestle at room temperature for short reaction time and easy operation under at room temperature. The meso substituted dipyrromethane is purified by crystallization and column chromatography o...

The Fenna-Matthews-Olson (FMO) protein complex was prepared from cells of the thermophilic green sulfur bacterium Chlorobium tepidum. A strain of C. tepidum that had been engineered to produce a His-tagged version of its reaction center was used in order to simplify the purification strategy. This strain was a gift of Prof. Oh-Oka (1). The cells were grown anaerobically in the light in modified...