the aim of this study was to investigate the in vitro developmental competence of isolated pre-antral follicles derived from vitrified ovaries in the presence of coenzyme q 10 (coq 10 ) . mice pre-antral follicles derived from fresh and vitrified-warmed ovarian tissues were in vitro cultured individually in α-mem medium supplemented with or without coq 10 , followed by adding human chorionic go...

background: human chorionic gonadotropin (hcg) has been used as a replacement for the mid-cycle luteinizing hormone (lh) surge for several years. the recent arrival of recombinant dna technology has made recombinant hcg (rhcg) accessible. objective: to assess efficacy of rhcg compared to urinary hcg (uhcg) for triggering of ovulation and induction of final oocyte maturation in assisted reproduc...

Cumulus cells are a group of closely associated granulosa cells that surround and nourish oocytes. Previous studies have shown that cumulus cells contribute to oocyte maturation and fertilization through gap junction communication. However, it is not known how this gap junction signaling affects in vivo versus in vitro maturation of oocytes, and their subsequent fertilization and embryonic deve...

Background: Cryopreservation of ovarian tissue is an important option for preserving the fertility of cancer patients undergoing chemotherapy and radiotherapy. Researchers have used different cryoprotectants and various techniques to improve the cryopreservation of ovaries However, despite significant recent progress, the efficiency of ovary cryopreservation is still low. Zinc is essential for ...

Oocyte maturation is a complex process involving nuclear and cytoplasmic maturation. The nuclear maturation is a chromosomal segregation and the cytoplasmic maturation involves the reorganization of the cytoplasmic organelles, mRNA transcription and storage of proteins to be used during fertilization and early embryo development. The mechanism of oocyte maturation in vivo and in vitro still are...

Oocytes of Pleurodeles waltl were activated after in vivo maturation by needle pricking or electric shock. After in vitro maturation, the oocytes were not activated by these stimuli. Coelomic oocytes and the oocytes which began their maturation in vivo could be activated by electric shock. During in vivo oocyte maturation, the activity of glucose-6-phosphate dehydrogenase (G6PDH), the key enzym...

sea cucumbers saponins are triterpenoid glycosides which exert beneficial biomedical effects. this study was performed to assess the effect of saponin extracted from sea cucumber holothuria leucospilota            ( h. leucospilota ) on maturation of mice oocytes and granulosa cells. the germinal vesicles oocytes were collected from 6–8 weeks old naval medical research institute (nmri) mice ova...

Leptin, the 16-kDa product of the obese (ob) gene, primarily secreted from adipose tissue, has been implicated to play an important role in the regulation of food intake and energy expenditure. This study investigated protective effect of leptin on trichostatin A-induced apoptotic on in vitro maturation ratio of buffalo oocytes. Ovaries were collected from abattoir and were transported...

BACKGROUND In spite of extensive efforts to improve in vitro oocyte maturation, the obtained results are not very efficient. This study was conducted to assess impacts of cAMP elevating agents and alpha lipoic acid (ALA) on in vitro oocyte maturation and fertilization. METHODS Mouse germinal vesicle (GV) oocytes were categorized into cumulus denuded oocytes (DOs; n=896) and cumulus oocyte com...